Detailed information of TA system TA216342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1712434..1713231
• Replicon: chromosome
• Accession: NZ_CP083403
• Organism: Enterobacter bugandensis strain XL95

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: K9O83_RS08090
• Protein ID: WP_172616463.1
• Coordinates: 1712731..1713231 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: K9O83_RS08085
• Protein ID: WP_008499829.1
• Coordinates: 1712434..1712703 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O83_RS08055 (K9O83_08050)	1708064..1708507	-	444	WP_224066473.1	helix-turn-helix domain-containing protein	-
K9O83_RS08060 (K9O83_08055)	1708660..1709901	+	1242	WP_224066474.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
K9O83_RS08065 (K9O83_08060)	1709935..1710162	-	228	WP_028012649.1	YccJ family protein	-
K9O83_RS08070 (K9O83_08065)	1710181..1710777	-	597	WP_041910554.1	NAD(P)H:quinone oxidoreductase	-
K9O83_RS08075 (K9O83_08070)	1711168..1711338	+	171	WP_001273664.1	general stress protein	-
K9O83_RS08080 (K9O83_08075)	1711455..1712372	+	918	WP_199886405.1	DMT family transporter	-
K9O83_RS08085 (K9O83_08080)	1712434..1712703	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
K9O83_RS08090 (K9O83_08085)	1712731..1713231	+	501	WP_172616463.1	GNAT family N-acetyltransferase	Toxin
K9O83_RS08095 (K9O83_08090)	1713290..1714612	-	1323	WP_224066475.1	pyrimidine utilization transport protein G	-
K9O83_RS08100 (K9O83_08095)	1714634..1715128	-	495	WP_063437641.1	pyrimidine utilization flavin reductase protein F	-
K9O83_RS08105 (K9O83_08100)	1715137..1715727	-	591	WP_041910551.1	malonic semialdehyde reductase	-
K9O83_RS08110 (K9O83_08105)	1715737..1716537	-	801	WP_048959640.1	pyrimidine utilization protein D	-
K9O83_RS08115 (K9O83_08110)	1716545..1716931	-	387	WP_224066476.1	pyrimidine utilization protein C	-
K9O83_RS08120 (K9O83_08115)	1716943..1717632	-	690	WP_193532723.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18873.63 Da
• Isoelectric Point: 8.4785

>T216342 WP_172616463.1 NZ_CP083403:1712731-1713231 [Enterobacter bugandensis]
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA

Nucleotide

• Length: 501 bp

>T216342 NZ_CP113018:760381-760647 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT216342 WP_008499829.1 NZ_CP083403:1712434-1712703 [Enterobacter bugandensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT216342 NZ_CP113018:760130-760381 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: W7PG43