Detailed information of TA system TA216335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 7910..8544
• Replicon: plasmid pT173d
• Accession: NZ_CP083374
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3R84_RS33520
• Protein ID: WP_057217370.1
• Coordinates: 8140..8544 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3R84_RS33515
• Protein ID: WP_025431366.1
• Coordinates: 7910..8140 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS33500 (J3R84_33280)	4662..6170	-	1509	WP_113569695.1	winged helix-turn-helix domain-containing tetratricopeptide repeat protein	-
J3R84_RS33505 (J3R84_33285)	6362..6577	+	216	WP_057217409.1	DUF1127 domain-containing protein	-
J3R84_RS33510 (J3R84_33290)	6590..7756	+	1167	WP_203529866.1	MFS transporter	-
J3R84_RS33515 (J3R84_33295)	7910..8140	+	231	WP_025431366.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J3R84_RS33520 (J3R84_33300)	8140..8544	+	405	WP_057217370.1	tRNA(fMet)-specific endonuclease VapC	Toxin
J3R84_RS33525 (J3R84_33305)	8646..8894	+	249	WP_025431368.1	DUF982 domain-containing protein	-
J3R84_RS33530 (J3R84_33310)	9294..9587	+	294	WP_057207789.1	WGR domain-containing protein	-
J3R84_RS33535 (J3R84_33315)	9643..9921	+	279	WP_025431370.1	HU family DNA-binding protein	-
J3R84_RS33540 (J3R84_33320)	10092..10310	-	219	WP_203529865.1	hypothetical protein	-
J3R84_RS33545 (J3R84_33325)	10339..12285	-	1947	WP_207207720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katB	1..946878	946878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15014.33 Da
• Isoelectric Point: 7.9129

>T216335 WP_057217370.1 NZ_CP083374:8140-8544 [Ensifer canadensis]
MLKYMLDTNICIFTIKNRPEPVREAFNRHQGQLCISSITLMELIFGAEKSAMPERNLNVVEGFAARLDVLHFDRLAAAHT
GQLRAELARAGKPIGPYDQLIAGHARSQGLILVTNNGREFERVPGLRLEDWATT

Nucleotide

• Length: 405 bp

>T216335 NZ_CP113016:c176152-175943 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8565.47 Da
• Isoelectric Point: 4.5258

>AT216335 WP_025431366.1 NZ_CP083374:7910-8140 [Ensifer canadensis]
MDQGSVFQSNRSQAIRLPKAVALPDDVKRVDVVAVGRTRIITPAGEAWDSWFNGERASADFMADRDQPEQQERETF

Nucleotide

• Length: 231 bp

>AT216335 NZ_CP113016:c176457-176164 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA