Detailed information of TA system TA216334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 564172..565679
• Replicon: plasmid pT173c
• Accession: NZ_CP083373
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: J3R84_RS32470
• Protein ID: WP_203529197.1
• Coordinates: 564441..565679 (+)
• Length: 413 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: J3R84_RS32465
• Protein ID: WP_057221040.1
• Coordinates: 564172..564444 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS32455 (J3R84_32255)	560126..562537	-	2412	WP_203529196.1	FtsX-like permease family protein	-
J3R84_RS32460 (J3R84_32260)	562534..563190	-	657	WP_057221043.1	ABC transporter ATP-binding protein	-
J3R84_RS32465 (J3R84_32265)	564172..564444	+	273	WP_057221040.1	helix-turn-helix domain-containing protein	Antitoxin
J3R84_RS32470 (J3R84_32270)	564441..565679	+	1239	WP_203529197.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3R84_RS32475 (J3R84_32275)	566107..566577	+	471	WP_203529199.1	GNAT family protein	-
J3R84_RS32480 (J3R84_32280)	567132..567374	-	243	WP_203529201.1	hypothetical protein	-
J3R84_RS32485 (J3R84_32285)	567696..568007	+	312	WP_203529203.1	hypothetical protein	-
J3R84_RS32490 (J3R84_32290)	568215..568835	-	621	WP_203529205.1	FMN-dependent NADH-azoreductase	-
J3R84_RS32495 (J3R84_32295)	569155..569763	+	609	WP_203529207.1	TetR/AcrR family transcriptional regulator	-
J3R84_RS32500 (J3R84_32300)	569870..570535	-	666	WP_203529209.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..782207	782207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 413 a.a.
• Molecular weight: 45145.04 Da
• Isoelectric Point: 8.1711

>T216334 WP_203529197.1 NZ_CP083373:564441-565679 [Ensifer canadensis]
MTTIFYETLPVARMTFEGEWRLDYDLSWKARRSAFPVSLTMPLRSGSVGADRLLPWLANLLPEAHLAEIGQRLKVSPQDI
VGLLGHIGRDTAGALSIGEARKAGVNHEPIPDEQALERILNELPAKPFLVGERGVSMSLAGVQEKLPVFVDEEGKISIPV
DGTPSTHIIKPDTRRLAGSVENEAFCLTLARACGLDAAETTIGAAGKRRYLLVKRYDRFTDPRGEIRRLHQEDLCQLTGH
FPSQKYERSSTGRGVTLKMMFGAVSDLVSPAERLKLLDAVIFNVLICNSDSHAKNYSILIGAAGSAKMAPLYDLMCAAVY
RQVDQSLPQAIAGRFHATDLRRSDWQELAKELGMSGASTIRRVEELAGLISSACEDVAPQIAAMAGDPTAVLVRIVHRVQ
KRCRRILAQAWG

Nucleotide

• Length: 1239 bp

>T216334 NZ_CP113016:c156565-156203 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9668.99 Da
• Isoelectric Point: 5.1367

>AT216334 WP_057221040.1 NZ_CP083373:564172-564444 [Ensifer canadensis]
MFRSAREFGAAVREKRKGLGWTQTELAARSGTGERFIVELESGKPSCQLEKALIVARTVGIEIGDLRAAQMTPTAPDDDL
GFLPTFGEGG

Nucleotide

• Length: 273 bp

>AT216334 NZ_CP113016:c156732-156562 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA