Detailed information of TA system TA216332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 233642..234312
• Replicon: plasmid pT173c
• Accession: NZ_CP083373
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3R84_RS30775
• Protein ID: WP_203529914.1
• Coordinates: 233893..234312 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3R84_RS30770
• Protein ID: WP_057220453.1
• Coordinates: 233642..233896 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS30760 (J3R84_30585)	228988..229977	-	990	WP_203529912.1	hypothetical protein	-
J3R84_RS30765 (J3R84_30590)	229977..232721	-	2745	WP_203529913.1	DUF499 domain-containing protein	-
J3R84_RS30770 (J3R84_30595)	233642..233896	+	255	WP_057220453.1	hypothetical protein	Antitoxin
J3R84_RS30775 (J3R84_30600)	233893..234312	+	420	WP_203529914.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3R84_RS30780 (J3R84_30605)	234446..235189	-	744	WP_057210789.1	3-keto-5-aminohexanoate cleavage protein	-
J3R84_RS30785 (J3R84_30610)	235892..236737	-	846	WP_203530047.1	LysR family transcriptional regulator	-
J3R84_RS30790 (J3R84_30615)	236838..237299	+	462	WP_057210749.1	RidA family protein	-
J3R84_RS30795 (J3R84_30620)	237311..238468	+	1158	WP_203530048.1	aminotransferase class V-fold PLP-dependent enzyme	-
J3R84_RS30800 (J3R84_30625)	238489..239277	+	789	WP_057210753.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..782207	782207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14767.80 Da
• Isoelectric Point: 4.5127

>T216332 WP_203529914.1 NZ_CP083373:233893-234312 [Ensifer canadensis]
MIVLDTNVVSEAMKPAPDLAIRNWLNDQVAETLYLSSVTLAELLFGIAALPEGRRKKALADTLDGLLELFDDRVLSFDTA
AARHYADLAATARAGGEGFPAPNGYIAAIAASKGFTIATRDTSPFEAAGVPVVNPWNHL

Nucleotide

• Length: 420 bp

>T216332 NZ_CP113015:c2964651-2964442 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9580.93 Da
• Isoelectric Point: 5.7485

>AT216332 WP_057220453.1 NZ_CP083373:233642-233896 [Ensifer canadensis]
MAILTVRNVPDEVHRALRVRAALHGRSTEAEVREILERAVKPEQRVRIGDALAELSGRLGLTNDDFEVFDQMREKAPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT216332 NZ_CP113015:c2964956-2964663 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA