Detailed information of TA system TA216331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 65532..66100
• Replicon: plasmid pT173c
• Accession: NZ_CP083373
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: J3R84_RS29900
• Protein ID: WP_057221702.1
• Coordinates: 65532..65777 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J3R84_RS29905
• Protein ID: WP_057221703.1
• Coordinates: 65774..66100 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS29875 (J3R84_29695)	60667..62202	-	1536	WP_203530156.1	IS21 family transposase	-
J3R84_RS29880 (J3R84_29700)	62374..63078	+	705	WP_207932906.1	NmrA family transcriptional regulator	-
J3R84_RS29885 (J3R84_29705)	63248..63436	-	189	WP_057205499.1	hypothetical protein	-
J3R84_RS29890 (J3R84_29710)	63726..64028	+	303	WP_203529848.1	helix-turn-helix transcriptional regulator	-
J3R84_RS29895 (J3R84_29715)	64021..65274	+	1254	WP_203529847.1	HipA domain-containing protein	-
J3R84_RS29900 (J3R84_29720)	65532..65777	+	246	WP_057221702.1	BrnT family toxin	Toxin
J3R84_RS29905 (J3R84_29725)	65774..66100	+	327	WP_057221703.1	BrnA antitoxin family protein	Antitoxin
J3R84_RS29910 (J3R84_29730)	66686..66994	+	309	WP_156408521.1	DUF1778 domain-containing protein	-
J3R84_RS29915 (J3R84_29735)	66994..67539	+	546	WP_057221705.1	hypothetical protein	-
J3R84_RS29920 (J3R84_29740)	67835..69856	-	2022	WP_225906534.1	methyl-accepting chemotaxis protein	-
J3R84_RS29925 (J3R84_29745)	70230..70631	-	402	WP_057221706.1	DUF1127 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..782207	782207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9107.50 Da
• Isoelectric Point: 8.6274

>T216331 WP_057221702.1 NZ_CP083373:65532-65777 [Ensifer canadensis]
MKITYDNHKRQSNIAKHGYDFDDLHGEFFMSSLVIPAKEGRFMAIGVLRNGVIAVVFAHLGTEGISIISMRDASQKERNL
L

Nucleotide

• Length: 246 bp

>T216331 NZ_CP113015:c2945048-2944686 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12368.99 Da
• Isoelectric Point: 9.9333

>AT216331 WP_057221703.1 NZ_CP083373:65774-66100 [Ensifer canadensis]
MTNQPRKVRYLSDKEEAEIQRKIASDPDAPEATDDQLAQARPFAEAFPDLAESIKRSRGRPAIEKPRQQISIRLDPDIIE
HYKRNCGKGWQSRLNDDLRKVVGLKKAS

Nucleotide

• Length: 327 bp

>AT216331 NZ_CP113015:c2945215-2945045 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA