Detailed information of TA system TA216330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 8364..9001
• Replicon: plasmid pT173b
• Accession: NZ_CP083372
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3R84_RS28545
• Protein ID: WP_203529924.1
• Coordinates: 8364..8765 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3R84_RS28550
• Protein ID: WP_088197974.1
• Coordinates: 8765..9001 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS28520 (J3R84_28370)	3610..3864	+	255	WP_239637596.1	single-stranded DNA-binding protein	-
J3R84_RS28525 (J3R84_28375)	4298..5146	+	849	WP_203530166.1	DUF932 domain-containing protein	-
J3R84_RS28530 (J3R84_28380)	5407..5853	+	447	WP_203530167.1	DNA-binding protein	-
J3R84_RS28540 (J3R84_28390)	6813..7271	+	459	WP_225968506.1	PIN domain-containing protein	-
J3R84_RS28545 (J3R84_28395)	8364..8765	-	402	WP_203529924.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3R84_RS28550 (J3R84_28400)	8765..9001	-	237	WP_088197974.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J3R84_RS28555 (J3R84_28405)	9574..10794	+	1221	WP_203529925.1	ParB/RepB/Spo0J family partition protein	-
J3R84_RS28560 (J3R84_28410)	10803..11489	+	687	WP_203529926.1	hypothetical protein	-
J3R84_RS28565 (J3R84_28415)	12407..12724	+	318	WP_203529927.1	DUF736 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..204278	204278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14846.06 Da
• Isoelectric Point: 5.1550

>T216330 WP_203529924.1 NZ_CP083372:c8765-8364 [Ensifer canadensis]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELDYGVSASDDPARELDNLAALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALNTVLVTNNTRDFLTYPGLKLENWLDT

Nucleotide

• Length: 402 bp

>T216330 NZ_CP113015:c2879894-2879499 [Staphylococcus aureus]
ATGCAAAACATTAAATATTTAACTGAAAAACAAGTAATTGCCATTAATGTAAAAGCAATTCAAGAACTCTCACCTAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAGGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTTGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCGGCTTTTATATACAGAAATATAGCTCAAAAACATGCATTCTTTAATGCGAATAAGAGA
ACAGCTTTTACATCTATGGTTATTTTCTTGAAGTTAAATAAGATTAATTTTAATTGTACTCAAGACGAAGCGGTACAATT
TACTTTAAAAGTGGTAGAGGATAAAACTCTTACACTTGAAGATATTGCAGATTGGATCAAAAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9023.09 Da
• Isoelectric Point: 5.6391

>AT216330 WP_088197974.1 NZ_CP083372:c9001-8765 [Ensifer canadensis]
MHTTKAFKNGNSQAVRIPAELAFDRTDVELEIERVGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQDERDKI

Nucleotide

• Length: 237 bp

>AT216330 NZ_CP113015:c2880124-2879894 [Staphylococcus aureus]
ATGGTTACTACAAGAAAACTAAGAAGAGCTGGCAATAGTTCAGTTGTTTCTGTACCAACAGAAGTGATCGCAGCACTCGG
AGTTACAAATGGTGACAATCTTAAATTTAATATTGAAGATAATAAAGTAACCATTGAAAAGGAGGTAAACGAAGACGAAG
AGTTTTTCAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTCTGAGAAAGTTGGTTGACATATAA