Detailed information of TA system TA216329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1486476..1487353
• Replicon: plasmid pT173e
• Accession: NZ_CP083371
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J3R84_RS26630
• Protein ID: WP_025428465.1
• Coordinates: 1486476..1486982 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J3R84_RS26635
• Protein ID: WP_025428464.1
• Coordinates: 1486982..1487353 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS26610 (J3R84_26485)	1482122..1482988	+	867	WP_225968517.1	sulfate ABC transporter permease subunit CysT	-
J3R84_RS26615 (J3R84_26490)	1482978..1483850	+	873	WP_084815706.1	sulfate ABC transporter permease subunit CysW	-
J3R84_RS26620 (J3R84_26495)	1483866..1484990	+	1125	WP_107027179.1	sulfate/molybdate ABC transporter ATP-binding protein	-
J3R84_RS26625 (J3R84_26500)	1485096..1486460	+	1365	WP_203529258.1	FAD-binding oxidoreductase	-
J3R84_RS26630 (J3R84_26505)	1486476..1486982	-	507	WP_025428465.1	RES domain-containing protein	Toxin
J3R84_RS26635 (J3R84_26510)	1486982..1487353	-	372	WP_025428464.1	DUF2384 domain-containing protein	Antitoxin
J3R84_RS26640 (J3R84_26515)	1487415..1488212	-	798	WP_203529280.1	crotonase/enoyl-CoA hydratase family protein	-
J3R84_RS26645 (J3R84_26520)	1488209..1489078	-	870	WP_057208936.1	hydroxymethylglutaryl-CoA lyase	-
J3R84_RS26650 (J3R84_26525)	1489075..1491063	-	1989	WP_203529283.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
J3R84_RS26655 (J3R84_26530)	1491080..1491463	-	384	WP_057208934.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..1913930	1913930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (8-157)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18694.11 Da
• Isoelectric Point: 5.1979

>T216329 WP_025428465.1 NZ_CP083371:c1486982-1486476 [Ensifer canadensis]
MSESEPVRLWRAYVPRWAHAPLSGDGAARFGGRWNPVGLPTIYAARELSTAWAEYNQSFVQHPALIAQLELRHAWLADMM
APDVLADFGVTDDIHREEWRDALDQGVVPATHRLQAELTSRGFDGLIYPSFMSPGGSCVALWRWNAGGAELTVIDPESRL
PKTPASWL

Nucleotide

• Length: 507 bp

>T216329 NZ_CP113015:c2773761-2773585 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTCATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13052.07 Da
• Isoelectric Point: 10.3367

>AT216329 WP_025428464.1 NZ_CP083371:c1487353-1486982 [Ensifer canadensis]
MSAVGFNISASHFGESQSSFLSARLVADRLGVTLAELAKLIGVARNTLTAKSSARKVDAALSKVVRILAMAAEMAGDESR
AVIWFKHQPIPGWAGKTAYDLAREGKADKVLAYLEAVRSGVYA

Nucleotide

• Length: 372 bp

>AT216329 NZ_CP113015:2773462-2773517 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG