Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35046..35716 Replicon plasmid p3
Accession NZ_CP083369
Organism Pseudomonas aeruginosa strain PS1793

Toxin (Protein)


Gene name vagD Uniprot ID Q2QCR9
Locus tag FLI66_RS35095 Protein ID WP_012477632.1
Coordinates 35279..35716 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q2QCS0
Locus tag FLI66_RS35090 Protein ID WP_012477631.1
Coordinates 35046..35282 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLI66_RS35065 30679..31083 + 405 WP_223271153.1 hypothetical protein -
FLI66_RS35070 (FLI66_35025) 31250..31867 + 618 WP_012477616.1 recombinase family protein -
FLI66_RS35075 (FLI66_35030) 31854..32990 + 1137 WP_012477617.1 replication initiator protein A -
FLI66_RS35080 (FLI66_35035) 33598..34248 + 651 WP_012477619.1 DNA-binding protein -
FLI66_RS35085 (FLI66_35040) 34611..35036 + 426 WP_015272397.1 DUF192 domain-containing protein -
FLI66_RS35090 (FLI66_35045) 35046..35282 + 237 WP_012477631.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FLI66_RS35095 (FLI66_35050) 35279..35716 + 438 WP_012477632.1 type II toxin-antitoxin system VapC family toxin Toxin
FLI66_RS35100 (FLI66_35055) 35723..36259 + 537 WP_053221386.1 hypothetical protein -
FLI66_RS35105 (FLI66_35060) 36274..37086 + 813 WP_054882063.1 3'-5' exonuclease -
FLI66_RS35110 (FLI66_35065) 37076..37384 + 309 WP_054882062.1 hypothetical protein -
FLI66_RS35115 (FLI66_35070) 37417..37656 + 240 WP_220467819.1 hypothetical protein -
FLI66_RS35120 (FLI66_35075) 38337..39173 + 837 WP_015272400.1 DUF932 domain-containing protein -
FLI66_RS35125 (FLI66_35080) 39229..39657 + 429 WP_015272401.1 hypothetical protein -
FLI66_RS35130 (FLI66_35085) 39674..40177 + 504 WP_054882242.1 hypothetical protein -
FLI66_RS35135 (FLI66_35090) 40238..40588 + 351 WP_046094857.1 TrfB-related DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..69506 69506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15671.07 Da        Isoelectric Point: 8.5199

>T216326 WP_012477632.1 NZ_CP083369:35279-35716 [Pseudomonas aeruginosa]
MSKKTYMLDTNICSFIMRERPDAVLAKLEQAVTNQHRIVVSAITYSEMRFGAANPKASPKVAAMVDAFIQRLDAILSWDA
AAVDQTTQIRTALARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPAITKS

Download         Length: 438 bp

>T216326 NZ_CP113015:c1815316-1814852 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGTTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAGTTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACACGGCTTTATCTCAGCTGTTCCATTACGTGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCGATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATTACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCATATTGATTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9070.29 Da        Isoelectric Point: 4.4563

>AT216326 WP_012477631.1 NZ_CP083369:35046-35282 [Pseudomonas aeruginosa]
MRTVSIFMNSRNQAIRIPRDMEYQGVSELEIIRDGETLILRPVRPSWASLRDVAAADPDFLTERKDVIEEDRFLGDFT

Download         Length: 237 bp

>AT216326 NZ_CP113015:c1815652-1815329 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A010RDD2

Antitoxin

Source ID Structure
AlphaFold DB A0A010SZE6

References