Detailed information of TA system TA216325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 13649..14196
• Replicon: plasmid p3
• Accession: NZ_CP083369
• Organism: Pseudomonas aeruginosa strain PS1793

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FLI66_RS34950
• Protein ID: WP_085286979.1
• Coordinates: 13649..13924 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FLI66_RS34955
• Protein ID: WP_085286980.1
• Coordinates: 13924..14196 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLI66_RS34925 (FLI66_34880)	8965..9291	+	327	WP_000091614.1	hypothetical protein	-
FLI66_RS34930 (FLI66_34885)	9288..9659	+	372	WP_023294894.1	hypothetical protein	-
FLI66_RS34935 (FLI66_34890)	9694..9804	+	111	Protein_12	thiol-disulfide isomerase	-
FLI66_RS34940 (FLI66_34895)	9801..10832	-	1032	WP_141989050.1	AraC family transcriptional regulator	-
FLI66_RS34945 (FLI66_34900)	10966..13350	+	2385	WP_141989052.1	penicillin acylase family protein	-
FLI66_RS34950 (FLI66_34905)	13649..13924	-	276	WP_085286979.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FLI66_RS34955 (FLI66_34910)	13924..14196	-	273	WP_085286980.1	ribbon-helix-helix domain-containing protein	Antitoxin
FLI66_RS34960 (FLI66_34915)	14218..14955	-	738	WP_141989053.1	DUF2807 domain-containing protein	-
FLI66_RS34965	15177..15554	-	378	WP_224397002.1	hypothetical protein	-
FLI66_RS34970 (FLI66_34925)	15681..16232	-	552	WP_141989055.1	hypothetical protein	-
FLI66_RS34975 (FLI66_34930)	16284..17315	-	1032	WP_054882046.1	ATPase, T2SS/T4P/T4SS family	-
FLI66_RS34980 (FLI66_34935)	17327..18484	-	1158	WP_054882047.1	type IV secretion system protein VirB10	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69506	69506

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10795.34 Da
• Isoelectric Point: 7.0128

>T216325 WP_085286979.1 NZ_CP083369:c13924-13649 [Pseudomonas aeruginosa]
MELKWTSKALSDITRLYEFLATVNQPAAARTVQQLTAAPTTLLTNPRIGERLEEFEPRDVRRIQIGQYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T216325 NZ_CP113015:1576321-1576614 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAAGTGTACTTAAAAGAGTTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCGTCACTTCAAGCCGATCAAAGCGAAAGTAACGCATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10091.37 Da
• Isoelectric Point: 5.6994

>AT216325 WP_085286980.1 NZ_CP083369:c14196-13924 [Pseudomonas aeruginosa]
MATTAKTRSVTAHVPEQLAQKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
SSDTPLPVPR

Nucleotide

• Length: 273 bp

>AT216325 NZ_CP113015:1576109-1576318 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA