216324

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 7898..8591 Replicon plasmid p3
Accession NZ_CP083369
Organism Pseudomonas aeruginosa strain PS1793

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag FLI66_RS34910 Protein ID WP_003151133.1
Coordinates 7898..8275 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag FLI66_RS34915 Protein ID WP_001172026.1
Coordinates 8256..8591 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLI66_RS34895 (FLI66_34850) 3216..4058 - 843 Protein_4 FAD-dependent oxidoreductase -
FLI66_RS34900 (FLI66_34855) 4091..7120 - 3030 WP_010465834.1 Tn3 family transposase -
FLI66_RS34905 (FLI66_34860) 7104..7706 - 603 WP_010465829.1 recombinase family protein -
FLI66_RS34910 (FLI66_34865) 7898..8275 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FLI66_RS34915 (FLI66_34870) 8256..8591 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
FLI66_RS34920 (FLI66_34875) 8606..8941 + 336 WP_000741275.1 hypothetical protein -
FLI66_RS34925 (FLI66_34880) 8965..9291 + 327 WP_000091614.1 hypothetical protein -
FLI66_RS34930 (FLI66_34885) 9288..9659 + 372 WP_023294894.1 hypothetical protein -
FLI66_RS34935 (FLI66_34890) 9694..9804 + 111 Protein_12 thiol-disulfide isomerase -
FLI66_RS34940 (FLI66_34895) 9801..10832 - 1032 WP_141989050.1 AraC family transcriptional regulator -
FLI66_RS34945 (FLI66_34900) 10966..13350 + 2385 WP_141989052.1 penicillin acylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..69506 69506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T216324 WP_003151133.1 NZ_CP083369:7898-8275 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T216324 NZ_CP113015:c1559180-1558992 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT216324 WP_001172026.1 NZ_CP083369:8256-8591 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT216324 NZ_CP113015:c1559469-1559209 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References