Detailed information of TA system TA216321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5598852..5599438
• Replicon: chromosome
• Accession: NZ_CP083366
• Organism: Pseudomonas aeruginosa strain PS1793

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: FLI66_RS26485
• Protein ID: WP_003120987.1
• Coordinates: 5599139..5599438 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FLI66_RS26480
• Protein ID: WP_003448662.1
• Coordinates: 5598852..5599142 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLI66_RS26460 (FLI66_26445)	5594003..5594212	+	210	WP_003105733.1	cold-shock protein	-
FLI66_RS26465 (FLI66_26450)	5594434..5596323	+	1890	WP_016851610.1	hypothetical protein	-
FLI66_RS26470 (FLI66_26455)	5596320..5598296	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
FLI66_RS35335	5598306..5598440	+	135	WP_255253641.1	hypothetical protein	-
FLI66_RS26475 (FLI66_26460)	5598437..5598781	+	345	WP_016851612.1	hypothetical protein	-
FLI66_RS26480 (FLI66_26465)	5598852..5599142	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
FLI66_RS26485 (FLI66_26470)	5599139..5599438	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FLI66_RS26490 (FLI66_26475)	5599640..5600764	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
FLI66_RS26495 (FLI66_26480)	5600764..5602473	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
FLI66_RS26500 (FLI66_26485)	5602477..5603802	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
FLI66_RS26505 (FLI66_26490)	5603792..5604325	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5571219..5659066	87847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T216321 WP_003120987.1 NZ_CP083366:c5599438-5599139 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T216321 NZ_CP113015:c239376-239110 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT216321 WP_003448662.1 NZ_CP083366:c5599142-5598852 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT216321 NZ_CP113015:c239627-239376 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: G8CP73