216320

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5174158..5174766 Replicon chromosome
Accession NZ_CP083366
Organism Pseudomonas aeruginosa strain PS1793

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag FLI66_RS24515 Protein ID WP_019486378.1
Coordinates 5174158..5174505 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag FLI66_RS24520 Protein ID WP_003114155.1
Coordinates 5174515..5174766 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLI66_RS24495 (FLI66_24485) 5170300..5171340 - 1041 WP_141989026.1 secretion protein HlyD -
FLI66_RS24500 (FLI66_24490) 5171368..5171793 - 426 WP_014819567.1 hypothetical protein -
FLI66_RS24505 (FLI66_24495) 5171981..5172589 - 609 WP_141989027.1 TetR/AcrR family transcriptional regulator -
FLI66_RS24510 (FLI66_24500) 5172873..5173769 + 897 WP_010792197.1 LysR family transcriptional regulator -
FLI66_RS24515 (FLI66_24505) 5174158..5174505 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FLI66_RS24520 (FLI66_24510) 5174515..5174766 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FLI66_RS24525 (FLI66_24515) 5174980..5175963 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
FLI66_RS24530 (FLI66_24520) 5175963..5177255 - 1293 WP_003115206.1 hypothetical protein -
FLI66_RS24535 (FLI66_24525) 5177514..5178776 - 1263 WP_019486376.1 zonular occludens toxin domain-containing protein -
FLI66_RS24540 (FLI66_24530) 5178778..5179128 - 351 WP_003133726.1 DUF2523 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element aph(3')-IIa / aph(6)-Ic - 5087961..5215004 127043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T216320 WP_019486378.1 NZ_CP083366:c5174505-5174158 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T216320 NZ_CP113009:c2301618-2301352 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT216320 WP_003114155.1 NZ_CP083366:c5174766-5174515 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT216320 NZ_CP113009:c2301875-2301618 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References