Detailed information of TA system TA216318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3672678..3673899
• Replicon: chromosome
• Accession: NZ_CP083366
• Organism: Pseudomonas aeruginosa strain PS1793

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: FLI66_RS17315
• Protein ID: WP_003110488.1
• Coordinates: 3673321..3673899 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FLI66_RS17310
• Protein ID: WP_003088112.1
• Coordinates: 3672678..3673232 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLI66_RS17285 (FLI66_17275)	3668246..3668797	+	552	WP_019485487.1	XRE family transcriptional regulator	-
FLI66_RS17290 (FLI66_17280)	3668794..3669192	-	399	WP_019485486.1	NADH-quinone oxidoreductase subunit A	-
FLI66_RS17295 (FLI66_17285)	3669205..3669528	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
FLI66_RS17300 (FLI66_17290)	3669859..3670320	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
FLI66_RS17305 (FLI66_17295)	3670325..3672520	+	2196	WP_019485485.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
FLI66_RS17310 (FLI66_17300)	3672678..3673232	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
FLI66_RS17315 (FLI66_17305)	3673321..3673899	+	579	WP_003110488.1	HD domain-containing protein	Toxin
FLI66_RS17320 (FLI66_17310)	3674009..3675196	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FLI66_RS17325 (FLI66_17315)	3675186..3677357	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
FLI66_RS17330 (FLI66_17320)	3677347..3678624	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T216318 WP_003110488.1 NZ_CP083366:3673321-3673899 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T216318 NZ_CP113009:c2244766-2244500 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT216318 WP_003088112.1 NZ_CP083366:3672678-3673232 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT216318 NZ_CP113009:c2245017-2244766 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4