Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 82091..82659 Replicon plasmid pVp-20-082E4A
Accession NZ_CP083363
Organism Vibrio parahaemolyticus strain 20-082E4

Toxin (Protein)


Gene name mazF Uniprot ID A0A1Y1BA95
Locus tag K9N54_RS25900 Protein ID WP_023624650.1
Coordinates 82091..82432 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7Y0S5I5
Locus tag K9N54_RS25905 Protein ID WP_031501799.1
Coordinates 82429..82659 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9N54_RS25875 (K9N54_25745) 77191..77829 + 639 Protein_78 IS5 family transposase -
K9N54_RS25880 (K9N54_25750) 78163..79239 + 1077 WP_086585381.1 replication initiation protein -
K9N54_RS25885 80130..80221 + 92 Protein_80 DUF3265 domain-containing protein -
K9N54_RS25890 (K9N54_25755) 80482..81213 + 732 WP_023624652.1 AHH domain-containing protein -
K9N54_RS25895 (K9N54_25760) 81213..81788 + 576 WP_017449240.1 hypothetical protein -
K9N54_RS25900 (K9N54_25765) 82091..82432 - 342 WP_023624650.1 endoribonuclease MazF Toxin
K9N54_RS25905 (K9N54_25770) 82429..82659 - 231 WP_031501799.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
K9N54_RS25910 (K9N54_25775) 83308..83835 + 528 WP_229036058.1 site-specific integrase -
K9N54_RS25915 (K9N54_25780) 83830..84213 - 384 Protein_86 recombinase family protein -
K9N54_RS25920 (K9N54_25785) 84816..85037 - 222 WP_023624649.1 hypothetical protein -
K9N54_RS25925 (K9N54_25790) 85347..85721 + 375 WP_042761155.1 hypothetical protein -
K9N54_RS25930 (K9N54_25795) 86282..87286 + 1005 WP_158501040.1 Ig-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..129872 129872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-107)

Antitoxin

Antitoxin PemI-like

(1-75)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12159.65 Da        Isoelectric Point: 5.8124

>T216313 WP_023624650.1 NZ_CP083363:c82432-82091 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Download         Length: 342 bp

>T216313 NZ_CP113009:c1731551-1731375 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTCATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8471.81 Da        Isoelectric Point: 5.6330

>AT216313 WP_031501799.1 NZ_CP083363:c82659-82429 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNQQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Download         Length: 231 bp

>AT216313 NZ_CP113009:1731252-1731307 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y1BA95

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y0S5I5

References