Detailed information of TA system TA216312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 3536973..3538321
• Replicon: chromosome
• Accession: NZ_CP083361
• Organism: Vibrio parahaemolyticus strain 20-082E4

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: K9N54_RS16990
• Protein ID: WP_156216669.1
• Coordinates: 3536973..3537914 (-)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A1R4LDG5
• Locus tag: K9N54_RS16995
• Protein ID: WP_000127617.1
• Coordinates: 3537917..3538321 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N54_RS16960 (K9N54_16840)	3533013..3533762	+	750	WP_156216627.1	SprT family zinc-dependent metalloprotease	-
K9N54_RS16965 (K9N54_16845)	3533788..3534384	+	597	WP_107111805.1	pancortin-3	-
K9N54_RS16970 (K9N54_16850)	3534384..3535496	+	1113	WP_156216625.1	hypothetical protein	-
K9N54_RS16975 (K9N54_16855)	3535535..3535747	-	213	WP_033929743.1	helix-turn-helix transcriptional regulator	-
K9N54_RS16980 (K9N54_16860)	3536051..3536326	+	276	WP_156216623.1	hypothetical protein	-
K9N54_RS16985 (K9N54_16865)	3536398..3536964	-	567	Protein_3245	type I restriction-modification system subunit M N-terminal domain-containing protein	-
K9N54_RS16990 (K9N54_16870)	3536973..3537914	-	942	WP_156216669.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K9N54_RS16995 (K9N54_16875)	3537917..3538321	-	405	WP_000127617.1	DUF6088 family protein	Antitoxin
K9N54_RS17000 (K9N54_16880)	3538583..3538771	-	189	WP_000342585.1	hypothetical protein	-
K9N54_RS17005 (K9N54_16885)	3538781..3539164	-	384	WP_156216668.1	hypothetical protein	-
K9N54_RS17010 (K9N54_16890)	3539157..3540500	-	1344	WP_156216666.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3501434..3547377	45943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-300)

Antitoxin

• Protein of unknown function DUF6088 (8-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35852.35 Da
• Isoelectric Point: 5.8951

>T216312 WP_156216669.1 NZ_CP083361:c3537914-3536973 [Vibrio parahaemolyticus]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLAELETLSVETHKLVFSGGTALAKSDVKILRMSEDVDIKLIPSQEFVV
LSRGKKKALRKALVEQIEARIGQSGHFAIEDKLVRDEYRYVEYELKYPQQFSQAPCLRPIIKLELMETISLHAIEERSIQ
SLVSELYQQSREVNAFACVSIQATLVEKVLSMLRRTMLVKRDSERNDDATLVRHIYDVHCIDIAEKTTSETLAPLFHTVL
QEDIQRFGNQHAEFVADPVNELNLGLQELESNPLFRQRFDDFVKPMVFNPQPHDFDTCFASFKRIAKSLITTI

Nucleotide

• Length: 942 bp

>T216312 NZ_CP113009:c1628891-1628739 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15292.58 Da
• Isoelectric Point: 10.3464

>AT216312 WP_000127617.1 NZ_CP083361:c3538321-3537917 [Vibrio parahaemolyticus]
MTAVECIYQKIKRSRRYVFERKDFDDVASYDQVGRALRQLVKQGDLIKIGYGLYTKAKLNSLTGKPMPTHPGGTDALMRE
ILKMKGVDFEMDSLSWQSLSGQSTQIPSSVQYSWNPKQFNRKLVVGNRVLNSQN

Nucleotide

• Length: 405 bp

>AT216312 NZ_CP113009:c1629515-1628916 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1R4LDG5