Detailed information of TA system TA021631

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3174300..3175135
• Replicon: chromosome
• Accession: NC_008253
• Organism: Escherichia coli 536

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: ECP_RS15390
• Protein ID: WP_001094445.1
• Coordinates: 3174758..3175135 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ECP_RS15385
• Protein ID: WP_024174331.1
• Coordinates: 3174300..3174668 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECP_RS15360	3171725..3171958	+	234	WP_001117568.1	DUF905 family protein	-
ECP_RS15365	3172049..3172867	+	819	WP_001234750.1	DUF945 domain-containing protein	-
ECP_RS15370	3172959..3173444	+	486	WP_000214415.1	antirestriction protein	-
ECP_RS15375	3173460..3173936	+	477	WP_001186725.1	RadC family protein	-
ECP_RS15380	3174005..3174226	+	222	WP_000692346.1	DUF987 domain-containing protein	-
ECP_RS15385	3174300..3174668	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECP_RS15390	3174758..3175135	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
ECP_RS15395	3175132..3175620	+	489	WP_000761718.1	hypothetical protein	-
ECP_RS15400	3175632..3175829	+	198	WP_000838536.1	DUF957 domain-containing protein	-
ECP_RS15405	3175914..3176756	+	843	WP_001280500.1	DUF4942 domain-containing protein	-
ECP_RS15410	3177099..3177269	+	171	Protein_2926	IS110 family transposase	-
ECP_RS15415	3178080..3179063	+	984	WP_001355482.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T21631 WP_001094445.1 NC_008253:3174758-3175135 [Escherichia coli 536]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T21631 NC_008253:3174758-3175135 [Escherichia coli 536]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT21631 WP_024174331.1 NC_008253:3174300-3174668 [Escherichia coli 536]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT21631 NC_008253:3174300-3174668 [Escherichia coli 536]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6