Detailed information of TA system TA216304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 164695..165275
• Replicon: plasmid p1
• Accession: NZ_CP083351
• Organism: Ensifer adhaerens strain W2-B9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC81_RS35410
• Protein ID: WP_223730869.1
• Coordinates: 164695..165078 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC81_RS35415
• Protein ID: WP_223730870.1
• Coordinates: 165075..165275 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC81_RS35390 (LAC81_35170)	160122..161465	+	1344	WP_223730866.1	glutamine synthetase family protein	-
LAC81_RS35395 (LAC81_35175)	161470..161976	+	507	WP_223730867.1	flavin reductase family protein	-
LAC81_RS35400 (LAC81_35180)	162053..163495	+	1443	WP_223730868.1	APC family permease	-
LAC81_RS35405 (LAC81_35185)	163500..164089	-	590	Protein_160	hypothetical protein	-
LAC81_RS35410 (LAC81_35190)	164695..165078	-	384	WP_223730869.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC81_RS35415 (LAC81_35195)	165075..165275	-	201	WP_223730870.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC81_RS35420 (LAC81_35200)	165827..166585	-	759	WP_235693208.1	HipA domain-containing protein	-
LAC81_RS35425 (LAC81_35205)	167211..167714	-	504	WP_223730872.1	hypothetical protein	-
LAC81_RS35430 (LAC81_35210)	168038..169039	+	1002	WP_223730873.1	IS30 family transposase	-
LAC81_RS35435 (LAC81_35215)	169141..169374	-	234	WP_223730874.1	hypothetical protein	-
LAC81_RS35440 (LAC81_35220)	169845..169994	-	150	WP_223730875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735709	735709
	-	flank	IS/Tn	-	-	168038..169039	1001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14063.21 Da
• Isoelectric Point: 6.6947

>T216304 WP_223730869.1 NZ_CP083351:c165078-164695 [Ensifer adhaerens]
VILADTSIWIDHFRHADGELRRIIEDDLLLCHPAVIGELALGSLRDRSNVLAVLAAQREALVATHDEVMTMIDRHAIFSM
GIGYTDAHLLASVLLDHRATLWTRDKRLRVAAEKAGALLHLAGNSRN

Nucleotide

• Length: 384 bp

>T216304 NZ_CP113007:1353710-1353862 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7093.20 Da
• Isoelectric Point: 11.4778

>AT216304 WP_223730870.1 NZ_CP083351:c165275-165075 [Ensifer adhaerens]
MRSTINLDDTLIERAKSLTGTKETSALVRQALETLVRVESGKRLIALGGTMPNAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT216304 NZ_CP113007:1353086-1353685 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA