Detailed information of TA system TA216302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 130775..131445
• Replicon: plasmid p1
• Accession: NZ_CP083351
• Organism: Ensifer adhaerens strain W2-B9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC81_RS35235
• Protein ID: WP_223730843.1
• Coordinates: 131026..131445 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC81_RS35230
• Protein ID: WP_223730842.1
• Coordinates: 130775..131029 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC81_RS35190 (LAC81_34975)	125860..126507	+	648	WP_223730835.1	nitrile hydratase subunit alpha	-
LAC81_RS35195 (LAC81_34980)	126507..127163	+	657	WP_223730836.1	nitrile hydratase subunit beta	-
LAC81_RS35205 (LAC81_34990)	127767..128147	-	381	WP_223730838.1	transcriptional regulator	-
LAC81_RS35210 (LAC81_34995)	128165..128476	-	312	WP_223730839.1	type II toxin-antitoxin system HigB family toxin	-
LAC81_RS35215 (LAC81_35000)	128671..129066	-	396	WP_223730840.1	hypothetical protein	-
LAC81_RS35220 (LAC81_35005)	129526..130386	-	861	Protein_125	IS30 family transposase	-
LAC81_RS35225 (LAC81_35010)	130412..130723	+	312	WP_223730841.1	hypothetical protein	-
LAC81_RS35230 (LAC81_35015)	130775..131029	+	255	WP_223730842.1	plasmid stabilization protein	Antitoxin
LAC81_RS35235 (LAC81_35020)	131026..131445	+	420	WP_223730843.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC81_RS35240 (LAC81_35025)	131792..132376	+	585	WP_223730844.1	helix-turn-helix domain-containing protein	-
LAC81_RS35245 (LAC81_35030)	132549..132872	+	324	WP_223730845.1	antibiotic biosynthesis monooxygenase family protein	-
LAC81_RS35250 (LAC81_35035)	132941..134953	+	2013	WP_223730846.1	hydantoinase/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735709	735709
	-	flank	IS/Tn	-	-	129526..130434	908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14875.05 Da
• Isoelectric Point: 4.4128

>T216302 WP_223730843.1 NZ_CP083351:131026-131445 [Ensifer adhaerens]
MIVLDTNVVSEAMKPAPDLAVRNWLNDQVAETLYLSSVTLAELLFGIAALPEGRRKKALAETLDGMLELFDDRVLPFDTA
AARHYADLAAIARAAGKGFPTPDGYIVAIAASRGFIIATRDTSPFEAAGLSVVNPWSDL

Nucleotide

• Length: 420 bp

>T216302 NZ_CP113007:1146252-1146647 [Staphylococcus aureus]
ATGCAAAACATTAAATATTTAACTGAAAAACAAGTAATTGCCATTAATGTAAAAGCAATTCAAGAACTCTCACCTAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAGGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTTGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCGGCTTTTATATACAGAAATATAGCTCAAAAACATGCATTCTTTAATGCGAATAAGAGA
ACAGCTTTTACATCTATGGTTATTTTCTTGAAGTTAAATAAGATTAATTTTAATTGTACTCAAGACGAAGCGGTACAATT
TACTTTAAAAGTGGTAGAGGATAAAACTCTTACACTTGAAGATATTGCAGATTGGATCAAAAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9576.93 Da
• Isoelectric Point: 5.7485

>AT216302 WP_223730842.1 NZ_CP083351:130775-131029 [Ensifer adhaerens]
MAILTVRNVPDEVHRALRVRAAIHGRSTEAEVREILERAVKPEQRVRIGDALAELSGRLGLTNEDFEVFDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT216302 NZ_CP113007:1146022-1146252 [Staphylococcus aureus]
ATGGTTACTACAAGAAAACTAAGAAGAGCTGGCAATAGTTCAGTTGTTTCTGTACCAACAGAAGTGATCGCAGCACTCGG
AGTTACAAATGGTGACAATCTTAAATTTAATATTGAAGATAATAAAGTAACCATTGAAAAGGAGGTAAACGAAGACGAAG
AGTTTTTCAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTCTGAGAAAGTTGGTTGACATATAA