Detailed information of TA system TA216301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 17520..18160
• Replicon: plasmid p1
• Accession: NZ_CP083351
• Organism: Ensifer adhaerens strain W2-B9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC81_RS34645
• Protein ID: WP_223730751.1
• Coordinates: 17520..17906 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC81_RS34650
• Protein ID: WP_223730752.1
• Coordinates: 17906..18160 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC81_RS34630 (LAC81_34450)	14641..15435	-	795	WP_223730749.1	hypothetical protein	-
LAC81_RS34635 (LAC81_34455)	16090..16482	+	393	WP_223730750.1	hypothetical protein	-
LAC81_RS34640	17028..17264	-	237	Protein_12	hypothetical protein	-
LAC81_RS34645 (LAC81_34460)	17520..17906	-	387	WP_223730751.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC81_RS34650 (LAC81_34465)	17906..18160	-	255	WP_223730752.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC81_RS34655	18319..18777	-	459	WP_223730753.1	type II toxin-antitoxin system VapC family toxin	-
LAC81_RS34660 (LAC81_34475)	18777..19019	-	243	WP_223730754.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LAC81_RS34665 (LAC81_34480)	19249..20898	+	1650	WP_223730755.1	Mu transposase C-terminal domain-containing protein	-
LAC81_RS34670 (LAC81_34485)	20889..21812	+	924	WP_223730756.1	TniB family NTP-binding protein	-
LAC81_RS38245	22220..22942	+	723	WP_235693206.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735709	735709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13924.65 Da
• Isoelectric Point: 4.3945

>T216301 WP_223730751.1 NZ_CP083351:c17906-17520 [Ensifer adhaerens]
MVIYTSVLAAIAFNEPEAATFHERIADDPVRLISAASVLEAAMVIETRLGEAAGADLDLWLYKAEVEIVPVTAEHADRAR
RAWRRYGEGRHPVSLNYGDCFSYALAALSGEPLLNKGSDFSQTDIDAA

Nucleotide

• Length: 387 bp

>T216301 NZ_CP113007:1081097-1081459 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9569.82 Da
• Isoelectric Point: 5.1862

>AT216301 WP_223730752.1 NZ_CP083351:c18160-17906 [Ensifer adhaerens]
MALSIKDHDTEQLARTLAERTGESITLATKRALEERLRRLGCDMRKAAFLEDLEAIQRRWNARPVLDSRTADEILGYDEN
GLPS

Nucleotide

• Length: 255 bp

>AT216301 NZ_CP113007:1080930-1081100 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA