Detailed information of TA system TA216299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 22247..22908
• Replicon: chromosome
• Accession: NZ_CP083350
• Organism: Ensifer adhaerens strain W2-B9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC81_RS20515
• Protein ID: WP_223729078.1
• Coordinates: 22504..22908 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC81_RS20510
• Protein ID: WP_223729077.1
• Coordinates: 22247..22507 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC81_RS20495 (LAC81_20440)	19141..20106	-	966	WP_223729076.1	IS481 family transposase	-
LAC81_RS20500 (LAC81_20445)	20448..20615	-	168	WP_223730445.1	hypothetical protein	-
LAC81_RS20505 (LAC81_20450)	20951..21274	-	324	WP_058323403.1	DUF736 family protein	-
LAC81_RS20510 (LAC81_20455)	22247..22507	+	261	WP_223729077.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC81_RS20515 (LAC81_20460)	22504..22908	+	405	WP_223729078.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC81_RS20520 (LAC81_20465)	23297..24487	+	1191	WP_223729079.1	DUF1173 domain-containing protein	-
LAC81_RS20525 (LAC81_20470)	24951..25868	-	918	WP_223729080.1	zincin-like metallopeptidase domain-containing protein	-
LAC81_RS38345	26698..26829	+	132	WP_268906830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	19141..20106	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-43)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14509.60 Da
• Isoelectric Point: 6.4819

>T216299 WP_223729078.1 NZ_CP083350:22504-22908 [Ensifer adhaerens]
VISHLLDTNAVIALIGRKSDRLVSRVLHSPQGSLGLPSVVAYELYFGAQKSAKVQHNLETLRLLMADFSILDFDQNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLHVEDWSV

Nucleotide

• Length: 405 bp

>T216299 NZ_CP113007:1004130-1004234 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9792.96 Da
• Isoelectric Point: 4.6510

>AT216299 WP_223729077.1 NZ_CP083350:22247-22507 [Ensifer adhaerens]
MPHLARVFQSGNSQAVRLPKEFRFNVERVEITQEGDALILRPHVEAGEPWSSLKAALARGMTDDFMESGREQPEQQDRPE
LDAVFQ

Nucleotide

• Length: 261 bp

>AT216299 NZ_CP113007:c1004393-1004229 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT