Detailed information of TA system TA216298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 274585..275141
• Replicon: plasmid p1
• Accession: NZ_CP083348
• Organism: Ensifer adhaerens strain W2-B

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LAC80_RS36020
• Protein ID: WP_223730964.1
• Coordinates: 274851..275141 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LAC80_RS36015
• Protein ID: WP_223730963.1
• Coordinates: 274585..274863 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC80_RS35985 (LAC80_35755)	269692..270462	+	771	WP_223730959.1	ABC transporter ATP-binding protein	-
LAC80_RS35990 (LAC80_35760)	270652..271707	-	1056	WP_223730960.1	LacI family DNA-binding transcriptional regulator	-
LAC80_RS35995 (LAC80_35765)	271716..271928	-	213	WP_223730961.1	tautomerase family protein	-
LAC80_RS36000 (LAC80_35770)	271944..272855	-	912	WP_223730962.1	SMP-30/gluconolactonase/LRE family protein	-
LAC80_RS36005 (LAC80_35775)	273206..273949	+	744	Protein_280	substrate-binding domain-containing protein	-
LAC80_RS36010 (LAC80_35780)	274184..274306	-	123	Protein_281	oligopeptide ABC transporter ATP-binding protein OppF	-
LAC80_RS38270	274295..274395	-	101	Protein_282	hypothetical protein	-
LAC80_RS36015 (LAC80_35785)	274585..274863	+	279	WP_223730963.1	CopG family ribbon-helix-helix protein	Antitoxin
LAC80_RS36020 (LAC80_35790)	274851..275141	+	291	WP_223730964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LAC80_RS36025 (LAC80_35795)	275463..275693	-	231	WP_057249963.1	30S ribosomal protein S21	-
LAC80_RS36030 (LAC80_35800)	275839..276633	-	795	WP_223730965.1	L,D-transpeptidase	-
LAC80_RS36035 (LAC80_35805)	276998..277561	-	564	WP_223730966.1	cytochrome b/b6 domain-containing protein	-
LAC80_RS36040 (LAC80_35810)	277558..277833	-	276	WP_223730967.1	PepSY domain-containing protein	-
LAC80_RS36045	277936..278313	+	378	WP_223730968.1	hypothetical protein	-
LAC80_RS36050 (LAC80_35820)	278310..278975	+	666	WP_223730969.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735984	735984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10925.62 Da
• Isoelectric Point: 9.4585

>T216298 WP_223730964.1 NZ_CP083348:274851-275141 [Ensifer adhaerens]
MPQIVFSSAAVRDLERLRKYLQPKSPLAAKRAAEAILKGVQALGAHPKMGRLIDDLPDQYREWLIDFGDSGYITRYRIDE
DVLTILAVRHQKESGF

Nucleotide

• Length: 291 bp

>T216298 NZ_CP113007:739288-739554 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10680.82 Da
• Isoelectric Point: 4.9565

>AT216298 WP_223730963.1 NZ_CP083348:274585-274863 [Ensifer adhaerens]
MASPTSLKLDDELKGRVQHLAEIRRRSSHWIMREAIAQYVEREEKRETLRQDTLDAWEEYQSAGLHATAAEVEKWLSTWG
TDDESAAPECHK

Nucleotide

• Length: 279 bp

>AT216298 NZ_CP113007:739037-739288 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA