Detailed information of TA system TA216297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 164695..165275
• Replicon: plasmid p1
• Accession: NZ_CP083348
• Organism: Ensifer adhaerens strain W2-B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC80_RS35415
• Protein ID: WP_223730869.1
• Coordinates: 164695..165078 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC80_RS35420
• Protein ID: WP_223730870.1
• Coordinates: 165075..165275 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC80_RS35395 (LAC80_35175)	160122..161465	+	1344	WP_223730866.1	glutamine synthetase family protein	-
LAC80_RS35400 (LAC80_35180)	161470..161976	+	507	WP_223730867.1	flavin reductase family protein	-
LAC80_RS35405 (LAC80_35185)	162053..163495	+	1443	WP_223730868.1	APC family permease	-
LAC80_RS35410 (LAC80_35190)	163500..164089	-	590	Protein_160	hypothetical protein	-
LAC80_RS35415 (LAC80_35195)	164695..165078	-	384	WP_223730869.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC80_RS35420 (LAC80_35200)	165075..165275	-	201	WP_223730870.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC80_RS35425 (LAC80_35205)	165827..166585	-	759	WP_235693208.1	HipA domain-containing protein	-
LAC80_RS35430 (LAC80_35210)	167211..167714	-	504	WP_223730872.1	hypothetical protein	-
LAC80_RS35435 (LAC80_35215)	168038..169039	+	1002	WP_223730873.1	IS30 family transposase	-
LAC80_RS35440 (LAC80_35220)	169141..169374	-	234	WP_223730874.1	hypothetical protein	-
LAC80_RS35445 (LAC80_35225)	169845..169994	-	150	WP_223730875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735984	735984
	-	flank	IS/Tn	-	-	168038..169039	1001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14063.21 Da
• Isoelectric Point: 6.6947

>T216297 WP_223730869.1 NZ_CP083348:c165078-164695 [Ensifer adhaerens]
VILADTSIWIDHFRHADGELRRIIEDDLLLCHPAVIGELALGSLRDRSNVLAVLAAQREALVATHDEVMTMIDRHAIFSM
GIGYTDAHLLASVLLDHRATLWTRDKRLRVAAEKAGALLHLAGNSRN

Nucleotide

• Length: 384 bp

>T216297 NZ_CP113007:714018-714125 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7093.20 Da
• Isoelectric Point: 11.4778

>AT216297 WP_223730870.1 NZ_CP083348:c165275-165075 [Ensifer adhaerens]
MRSTINLDDTLIERAKSLTGTKETSALVRQALETLVRVESGKRLIALGGTMPNAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT216297 NZ_CP113007:c714202-714142 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA