Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 130775..131445 Replicon plasmid p1
Accession NZ_CP083348
Organism Ensifer adhaerens strain W2-B

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LAC80_RS35240 Protein ID WP_223730843.1
Coordinates 131026..131445 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag LAC80_RS35235 Protein ID WP_223730842.1
Coordinates 130775..131029 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAC80_RS35195 (LAC80_34980) 125860..126507 + 648 WP_223730835.1 nitrile hydratase subunit alpha -
LAC80_RS35200 (LAC80_34985) 126507..127163 + 657 WP_223730836.1 nitrile hydratase subunit beta -
LAC80_RS35210 (LAC80_34995) 127767..128147 - 381 WP_223730838.1 transcriptional regulator -
LAC80_RS35215 (LAC80_35000) 128165..128476 - 312 WP_223730839.1 type II toxin-antitoxin system HigB family toxin -
LAC80_RS35220 (LAC80_35005) 128671..129066 - 396 WP_223730840.1 hypothetical protein -
LAC80_RS35225 (LAC80_35010) 129526..130386 - 861 Protein_125 IS30 family transposase -
LAC80_RS35230 (LAC80_35015) 130412..130723 + 312 WP_223730841.1 hypothetical protein -
LAC80_RS35235 (LAC80_35020) 130775..131029 + 255 WP_223730842.1 plasmid stabilization protein Antitoxin
LAC80_RS35240 (LAC80_35025) 131026..131445 + 420 WP_223730843.1 type II toxin-antitoxin system VapC family toxin Toxin
LAC80_RS35245 (LAC80_35030) 131792..132376 + 585 WP_223730844.1 helix-turn-helix domain-containing protein -
LAC80_RS35250 (LAC80_35035) 132549..132872 + 324 WP_223730845.1 antibiotic biosynthesis monooxygenase family protein -
LAC80_RS35255 (LAC80_35040) 132941..134953 + 2013 WP_223730846.1 hydantoinase/oxoprolinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..735984 735984
- flank IS/Tn - - 129526..130434 908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14875.05 Da        Isoelectric Point: 4.4128

>T216295 WP_223730843.1 NZ_CP083348:131026-131445 [Ensifer adhaerens]
MIVLDTNVVSEAMKPAPDLAVRNWLNDQVAETLYLSSVTLAELLFGIAALPEGRRKKALAETLDGMLELFDDRVLPFDTA
AARHYADLAAIARAAGKGFPTPDGYIVAIAASRGFIIATRDTSPFEAAGLSVVNPWSDL

Download         Length: 420 bp

>T216295 NZ_CP113005:c2130275-2130009 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9576.93 Da        Isoelectric Point: 5.7485

>AT216295 WP_223730842.1 NZ_CP083348:130775-131029 [Ensifer adhaerens]
MAILTVRNVPDEVHRALRVRAAIHGRSTEAEVREILERAVKPEQRVRIGDALAELSGRLGLTNEDFEVFDQVRDKVPAEP
MRFE

Download         Length: 255 bp

>AT216295 NZ_CP113005:c2130532-2130275 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References