Detailed information of TA system TA216294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 17520..18160
• Replicon: plasmid p1
• Accession: NZ_CP083348
• Organism: Ensifer adhaerens strain W2-B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC80_RS34650
• Protein ID: WP_223730751.1
• Coordinates: 17520..17906 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC80_RS34655
• Protein ID: WP_223730752.1
• Coordinates: 17906..18160 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC80_RS34635 (LAC80_34455)	14641..15435	-	795	WP_223730749.1	hypothetical protein	-
LAC80_RS34640 (LAC80_34460)	16090..16482	+	393	WP_223730750.1	hypothetical protein	-
LAC80_RS34645	17028..17264	-	237	Protein_12	hypothetical protein	-
LAC80_RS34650 (LAC80_34465)	17520..17906	-	387	WP_223730751.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC80_RS34655 (LAC80_34470)	17906..18160	-	255	WP_223730752.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC80_RS34660	18319..18777	-	459	WP_223730753.1	type II toxin-antitoxin system VapC family toxin	-
LAC80_RS34665 (LAC80_34480)	18777..19019	-	243	WP_223730754.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LAC80_RS34670 (LAC80_34485)	19249..20898	+	1650	WP_223730755.1	Mu transposase C-terminal domain-containing protein	-
LAC80_RS34675 (LAC80_34490)	20889..21812	+	924	WP_223730756.1	TniB family NTP-binding protein	-
LAC80_RS38265	22220..22942	+	723	WP_235693206.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735984	735984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13924.65 Da
• Isoelectric Point: 4.3945

>T216294 WP_223730751.1 NZ_CP083348:c17906-17520 [Ensifer adhaerens]
MVIYTSVLAAIAFNEPEAATFHERIADDPVRLISAASVLEAAMVIETRLGEAAGADLDLWLYKAEVEIVPVTAEHADRAR
RAWRRYGEGRHPVSLNYGDCFSYALAALSGEPLLNKGSDFSQTDIDAA

Nucleotide

• Length: 387 bp

>T216294 NZ_CP113005:c2098693-2098586 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9569.82 Da
• Isoelectric Point: 5.1862

>AT216294 WP_223730752.1 NZ_CP083348:c18160-17906 [Ensifer adhaerens]
MALSIKDHDTEQLARTLAERTGESITLATKRALEERLRRLGCDMRKAAFLEDLEAIQRRWNARPVLDSRTADEILGYDEN
GLPS

Nucleotide

• Length: 255 bp

>AT216294 NZ_CP113005:2098509-2098569 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA