Detailed information of TA system TA216293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2443949..2444610
• Replicon: chromosome
• Accession: NZ_CP083347
• Organism: Ensifer adhaerens strain W2-B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAC80_RS31100
• Protein ID: WP_223729078.1
• Coordinates: 2444206..2444610 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LAC80_RS31095
• Protein ID: WP_223729077.1
• Coordinates: 2443949..2444209 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC80_RS31080 (LAC80_30955)	2440843..2441808	-	966	WP_223729076.1	IS481 family transposase	-
LAC80_RS31085 (LAC80_30960)	2442150..2442317	-	168	WP_223730445.1	hypothetical protein	-
LAC80_RS31090 (LAC80_30965)	2442653..2442976	-	324	WP_058323403.1	DUF736 family protein	-
LAC80_RS31095 (LAC80_30970)	2443949..2444209	+	261	WP_223729077.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LAC80_RS31100 (LAC80_30975)	2444206..2444610	+	405	WP_223729078.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAC80_RS31105 (LAC80_30980)	2444999..2446189	+	1191	WP_223729079.1	DUF1173 domain-containing protein	-
LAC80_RS31110 (LAC80_30985)	2446653..2447570	-	918	WP_223729080.1	zincin-like metallopeptidase domain-containing protein	-
LAC80_RS38385	2448400..2448531	+	132	WP_268906830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2440843..2441808	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-43)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14509.60 Da
• Isoelectric Point: 6.4819

>T216293 WP_223729078.1 NZ_CP083347:2444206-2444610 [Ensifer adhaerens]
VISHLLDTNAVIALIGRKSDRLVSRVLHSPQGSLGLPSVVAYELYFGAQKSAKVQHNLETLRLLMADFSILDFDQNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLHVEDWSV

Nucleotide

• Length: 405 bp

>T216293 NZ_CP113005:c2073423-2073157 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9792.96 Da
• Isoelectric Point: 4.6510

>AT216293 WP_223729077.1 NZ_CP083347:2443949-2444209 [Ensifer adhaerens]
MPHLARVFQSGNSQAVRLPKEFRFNVERVEITQEGDALILRPHVEAGEPWSSLKAALARGMTDDFMESGREQPEQQDRPE
LDAVFQ

Nucleotide

• Length: 261 bp

>AT216293 NZ_CP113005:c2073674-2073423 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA