Detailed information of TA system TA216291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 274585..275141
• Replicon: plasmid p1
• Accession: NZ_CP083345
• Organism: Ensifer adhaerens strain W2-A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LAC78_RS37350
• Protein ID: WP_223730964.1
• Coordinates: 274851..275141 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LAC78_RS37345
• Protein ID: WP_223730963.1
• Coordinates: 274585..274863 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC78_RS37315 (LAC78_37050)	269692..270462	+	771	WP_223730959.1	ABC transporter ATP-binding protein	-
LAC78_RS37320 (LAC78_37055)	270652..271707	-	1056	WP_223730960.1	LacI family DNA-binding transcriptional regulator	-
LAC78_RS37325 (LAC78_37060)	271716..271928	-	213	WP_223730961.1	tautomerase family protein	-
LAC78_RS37330 (LAC78_37065)	271944..272855	-	912	WP_223730962.1	SMP-30/gluconolactonase/LRE family protein	-
LAC78_RS37335 (LAC78_37070)	273206..273949	+	744	Protein_280	substrate-binding domain-containing protein	-
LAC78_RS37340 (LAC78_37075)	274184..274306	-	123	Protein_281	oligopeptide ABC transporter ATP-binding protein OppF	-
LAC78_RS39600	274295..274395	-	101	Protein_282	hypothetical protein	-
LAC78_RS37345 (LAC78_37080)	274585..274863	+	279	WP_223730963.1	CopG family ribbon-helix-helix protein	Antitoxin
LAC78_RS37350 (LAC78_37085)	274851..275141	+	291	WP_223730964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LAC78_RS37355 (LAC78_37090)	275463..275693	-	231	WP_057249963.1	30S ribosomal protein S21	-
LAC78_RS37360 (LAC78_37095)	275839..276633	-	795	WP_223730965.1	L,D-transpeptidase	-
LAC78_RS37365 (LAC78_37100)	276998..277561	-	564	WP_223730966.1	cytochrome b/b6 domain-containing protein	-
LAC78_RS37370 (LAC78_37105)	277558..277833	-	276	WP_223730967.1	PepSY domain-containing protein	-
LAC78_RS37375	277936..278313	+	378	WP_223730968.1	hypothetical protein	-
LAC78_RS37380 (LAC78_37115)	278310..278975	+	666	WP_223730969.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735984	735984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10925.62 Da
• Isoelectric Point: 9.4585

>T216291 WP_223730964.1 NZ_CP083345:274851-275141 [Ensifer adhaerens]
MPQIVFSSAAVRDLERLRKYLQPKSPLAAKRAAEAILKGVQALGAHPKMGRLIDDLPDQYREWLIDFGDSGYITRYRIDE
DVLTILAVRHQKESGF

Nucleotide

• Length: 291 bp

>T216291 NZ_CP113005:c1751098-1750889 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10680.82 Da
• Isoelectric Point: 4.9565

>AT216291 WP_223730963.1 NZ_CP083345:274585-274863 [Ensifer adhaerens]
MASPTSLKLDDELKGRVQHLAEIRRRSSHWIMREAIAQYVEREEKRETLRQDTLDAWEEYQSAGLHATAAEVEKWLSTWG
TDDESAAPECHK

Nucleotide

• Length: 279 bp

>AT216291 NZ_CP113005:c1751403-1751110 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA