Detailed information of TA system TA216282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4304471..4305285
• Replicon: chromosome
• Accession: NZ_CP083274
• Organism: Escherichia coli strain Rosetta2-DE3

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K6U01_RS20740
• Protein ID: WP_001054376.1
• Coordinates: 4304471..4304728 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K6U01_RS20745
• Protein ID: WP_001309181.1
• Coordinates: 4304740..4305285 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6U01_RS20715 (4299759)	4299759..4300865	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
K6U01_RS20720 (4300930)	4300930..4301910	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K6U01_RS20725 (4302020)	4302020..4302225	+	206	Protein_4083	HNH endonuclease	-
K6U01_RS20730 (4302493)	4302493..4303733	-	1241	Protein_4084	helicase YjhR	-
K6U01_RS20735 (4303849)	4303849..4303980	+	132	WP_001309182.1	hypothetical protein	-
K6U01_RS20740 (4304471)	4304471..4304728	+	258	WP_001054376.1	YjhX family toxin	Toxin
K6U01_RS20745 (4304740)	4304740..4305285	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K6U01_RS20750 (4305341)	4305341..4306087	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K6U01_RS20755 (4306256)	4306256..4306474	+	219	Protein_4089	hypothetical protein	-
K6U01_RS20760 (4306512)	4306512..4306628	+	117	Protein_4090	VOC family protein	-
K6U01_RS20765 (4306873)	4306873..4307994	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K6U01_RS20770 (4307991)	4307991..4308269	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K6U01_RS20775 (4308281)	4308281..4309594	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T216282 WP_001054376.1 NZ_CP083274:4304471-4304728 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T216282 NZ_CP112997:c3752744-3752352 [Mycobacterium tuberculosis variant bovis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT216282 WP_001309181.1 NZ_CP083274:4304740-4305285 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT216282 NZ_CP112997:c3753019-3752744 [Mycobacterium tuberculosis variant bovis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8