Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3748296..3748934 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag K6U01_RS18025 Protein ID WP_000813794.1
Coordinates 3748296..3748472 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag K6U01_RS18030 Protein ID WP_001270286.1
Coordinates 3748518..3748934 (+) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS18005 (3743915) 3743915..3745129 - 1215 WP_001326689.1 BenE family transporter YdcO -
K6U01_RS18010 (3745182) 3745182..3745718 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
K6U01_RS18015 (3745791) 3745791..3747752 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
K6U01_RS18020 (3747844) 3747844..3748074 - 231 WP_000494244.1 YncJ family protein -
K6U01_RS18025 (3748296) 3748296..3748472 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
K6U01_RS18030 (3748518) 3748518..3748934 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
K6U01_RS18035 (3749013) 3749013..3750419 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
K6U01_RS18040 (3750664) 3750664..3751809 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
K6U01_RS18045 (3751827) 3751827..3752840 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
K6U01_RS18050 (3752841) 3752841..3753782 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-57)

Antitoxin

(91-134)


Sequences


Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T216279 WP_000813794.1 NZ_CP083274:3748296-3748472 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T216279 NZ_CP112997:3510768-3511112 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT216279 WP_001270286.1 NZ_CP083274:3748518-3748934 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT216279 NZ_CP112997:3511109-3511438 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6HPB


Antitoxin

Source ID Structure

References