216277

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3083266..3084103 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag K6U01_RS14795 Protein ID WP_000227786.1
Coordinates 3083561..3084103 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag K6U01_RS14790 Protein ID WP_001297137.1
Coordinates 3083266..3083577 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS14765 (3078286) 3078286..3079233 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
K6U01_RS14770 (3079255) 3079255..3081246 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
K6U01_RS14775 (3081236) 3081236..3081850 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
K6U01_RS14780 (3081850) 3081850..3082179 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
K6U01_RS14785 (3082191) 3082191..3083081 + 891 WP_000971336.1 heme o synthase -
K6U01_RS14790 (3083266) 3083266..3083577 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
K6U01_RS14795 (3083561) 3083561..3084103 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
K6U01_RS14800 (3084159) 3084159..3085094 - 936 WP_001297127.1 tetratricopeptide repeat protein -
K6U01_RS14805 (3085511) 3085511..3086875 + 1365 WP_001000978.1 MFS transporter -
K6U01_RS14810 (3087003) 3087003..3087494 - 492 WP_001138904.1 nucleotide binding protein YajQ -
K6U01_RS14815 (3087662) 3087662..3088573 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T216277 WP_000227786.1 NZ_CP083274:3083561-3084103 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T216277 NZ_CP112997:3137234-3137497 [Mycobacterium tuberculosis variant bovis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT216277 WP_001297137.1 NZ_CP083274:3083266-3083577 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT216277 NZ_CP112997:3136949-3137230 [Mycobacterium tuberculosis variant bovis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References