Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2753339..2753836 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K6U01_RS13225 Protein ID WP_001173343.1
Coordinates 2753339..2753512 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K6U01_RS13230 Protein ID WP_000398619.1
Coordinates 2753540..2753836 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS13215 (2750009) 2750009..2751466 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
K6U01_RS13220 (2751703) 2751703..2753220 + 1518 WP_001295090.1 lysine--tRNA ligase -
K6U01_RS13225 (2753339) 2753339..2753512 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K6U01_RS13230 (2753540) 2753540..2753836 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K6U01_RS13235 (2754063) 2754063..2754335 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
K6U01_RS13240 (2754347) 2754347..2754577 - 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
K6U01_RS13245 (2754758) 2754758..2756394 + 1637 Protein_2615 sensor histidine kinase -
K6U01_RS13250 (2756391) 2756391..2757110 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216274 WP_001173343.1 NZ_CP083274:c2753512-2753339 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216274 NZ_CP112997:c3069974-3069618 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216274 WP_000398619.1 NZ_CP083274:c2753836-2753540 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216274 NZ_CP112997:c3070188-3069958 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References