Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2116821..2117652 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag ECP_RS10435 Protein ID WP_000854814.1
Coordinates 2117278..2117652 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag ECP_RS10430 Protein ID WP_001285586.1
Coordinates 2116821..2117189 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS10390 2111878..2112948 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
ECP_RS10395 2113084..2113761 + 678 WP_001362823.1 hypothetical protein -
ECP_RS10400 2113777..2114187 + 411 WP_000846704.1 hypothetical protein -
ECP_RS10405 2114408..2115229 + 822 WP_001234571.1 DUF945 domain-containing protein -
ECP_RS10415 2115492..2115965 + 474 WP_011579143.1 antirestriction protein -
ECP_RS10420 2115981..2116457 + 477 WP_011579144.1 RadC family protein -
ECP_RS10425 2116526..2116747 + 222 WP_000692323.1 DUF987 domain-containing protein -
ECP_RS10430 2116821..2117189 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ECP_RS10435 2117278..2117652 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
ECP_RS10440 2117649..2117843 + 195 WP_000988601.1 hypothetical protein -
ECP_RS26915 2117856..2117969 + 114 WP_001161660.1 DUF957 domain-containing protein -
ECP_RS25035 2118258..2118404 - 147 Protein_1982 transposase domain-containing protein -
ECP_RS10450 2118458..2118640 + 183 WP_001016348.1 hypothetical protein -
ECP_RS10455 2118741..2119070 - 330 WP_000450409.1 DUF496 family protein -
ECP_RS10460 2119242..2120300 - 1059 WP_001200890.1 FUSC family protein -
ECP_RS10465 2120498..2120971 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
ECP_RS10470 2121090..2122256 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T21627 WP_000854814.1 NC_008253:2117278-2117652 [Escherichia coli 536]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T21627 NC_008253:2117278-2117652 [Escherichia coli 536]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT21627 WP_001285586.1 NC_008253:2116821-2117189 [Escherichia coli 536]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT21627 NC_008253:2116821-2117189 [Escherichia coli 536]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References