Detailed information of TA system TA216254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3834367..3835005
• Replicon: chromosome
• Accession: NZ_CP083273
• Organism: Escherichia coli strain BL21-RIPL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K9U36_RS18565
• Protein ID: WP_000813794.1
• Coordinates: 3834367..3834543 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U36_RS18570
• Protein ID: WP_001270286.1
• Coordinates: 3834589..3835005 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U36_RS18545 (3829986)	3829986..3831200	-	1215	WP_001326689.1	BenE family transporter YdcO	-
K9U36_RS18550 (3831253)	3831253..3831789	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K9U36_RS18555 (3831862)	3831862..3833823	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U36_RS18560 (3833915)	3833915..3834145	-	231	WP_000494244.1	YncJ family protein	-
K9U36_RS18565 (3834367)	3834367..3834543	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U36_RS18570 (3834589)	3834589..3835005	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U36_RS18575 (3835084)	3835084..3836490	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K9U36_RS18580 (3836735)	3836735..3837880	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K9U36_RS18585 (3837898)	3837898..3838911	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K9U36_RS18590 (3838912)	3838912..3839853	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T216254 WP_000813794.1 NZ_CP083273:3834367-3834543 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216254 NZ_CP112997:c1935521-1935132 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216254 WP_001270286.1 NZ_CP083273:3834589-3835005 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216254 NZ_CP112997:c1935745-1935518 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• PDB: 6HPB