216252

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3007270..3008107 Replicon chromosome
Accession NZ_CP083273
Organism Escherichia coli strain BL21-RIPL

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag K9U36_RS14440 Protein ID WP_000227786.1
Coordinates 3007565..3008107 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag K9U36_RS14435 Protein ID WP_001297137.1
Coordinates 3007270..3007581 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U36_RS14410 (3002290) 3002290..3003237 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
K9U36_RS14415 (3003259) 3003259..3005250 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
K9U36_RS14420 (3005240) 3005240..3005854 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
K9U36_RS14425 (3005854) 3005854..3006183 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
K9U36_RS14430 (3006195) 3006195..3007085 + 891 WP_000971336.1 heme o synthase -
K9U36_RS14435 (3007270) 3007270..3007581 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
K9U36_RS14440 (3007565) 3007565..3008107 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
K9U36_RS14445 (3008163) 3008163..3009098 - 936 WP_001297127.1 tetratricopeptide repeat protein -
K9U36_RS14450 (3009515) 3009515..3010879 + 1365 WP_001000978.1 MFS transporter -
K9U36_RS14455 (3011007) 3011007..3011498 - 492 WP_001138904.1 nucleotide binding protein YajQ -
K9U36_RS14460 (3011666) 3011666..3012577 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T216252 WP_000227786.1 NZ_CP083273:3007565-3008107 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T216252 NZ_CP112997:1686079-1686396 [Mycobacterium tuberculosis variant bovis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT216252 WP_001297137.1 NZ_CP083273:3007270-3007581 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT216252 NZ_CP112997:1685780-1686082 [Mycobacterium tuberculosis variant bovis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References