Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2626570..2627067 Replicon chromosome
Accession NZ_CP083273
Organism Escherichia coli strain BL21-RIPL

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9U36_RS12630 Protein ID WP_001173343.1
Coordinates 2626570..2626743 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K9U36_RS12635 Protein ID WP_000398619.1
Coordinates 2626771..2627067 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U36_RS12620 (2623240) 2623240..2624697 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
K9U36_RS12625 (2624934) 2624934..2626451 + 1518 WP_001295090.1 lysine--tRNA ligase -
K9U36_RS12630 (2626570) 2626570..2626743 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9U36_RS12635 (2626771) 2626771..2627067 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9U36_RS12640 (2627294) 2627294..2627566 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
K9U36_RS12645 (2627578) 2627578..2627808 - 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
K9U36_RS12650 (2627989) 2627989..2629625 + 1637 Protein_2498 sensor histidine kinase -
K9U36_RS12655 (2629622) 2629622..2630341 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216249 WP_001173343.1 NZ_CP083273:c2626743-2626570 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216249 NZ_CP112997:c1388586-1388293 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216249 WP_000398619.1 NZ_CP083273:c2627067-2626771 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216249 NZ_CP112997:c1388852-1388583 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References