Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1863586..1864240 Replicon chromosome
Accession NZ_CP083273
Organism Escherichia coli strain BL21-RIPL

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag K9U36_RS08925 Protein ID WP_000244783.1
Coordinates 1863833..1864240 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K9U36_RS08920 Protein ID WP_000354046.1
Coordinates 1863586..1863852 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U36_RS08895 (1858755) 1858755..1859498 + 744 WP_000951948.1 SDR family oxidoreductase -
K9U36_RS08900 (1859555) 1859555..1860988 - 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
K9U36_RS08905 (1861033) 1861033..1861344 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K9U36_RS08910 (1861508) 1861508..1862167 + 660 WP_000250274.1 hemolysin III family protein -
K9U36_RS08915 (1862363) 1862363..1863343 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K9U36_RS08920 (1863586) 1863586..1863852 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K9U36_RS08925 (1863833) 1863833..1864240 + 408 WP_000244783.1 protein YgfX Toxin
K9U36_RS08930 (1864280) 1864280..1864801 - 522 WP_001055874.1 flavodoxin FldB -
K9U36_RS08935 (1864913) 1864913..1865809 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K9U36_RS08940 (1865834) 1865834..1866544 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K9U36_RS08945 (1866550) 1866550..1868283 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T216247 WP_000244783.1 NZ_CP083273:1863833-1864240 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216247 NZ_CP112997:1239277-1239651 [Mycobacterium tuberculosis variant bovis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216247 WP_000354046.1 NZ_CP083273:1863586-1863852 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216247 NZ_CP112997:1239083-1239280 [Mycobacterium tuberculosis variant bovis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References