Detailed information of TA system TA216236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200570..201206
• Replicon: chromosome
• Accession: NZ_CP083269
• Organism: Priestia aryabhattai strain NM1_A2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: K8Z47_RS01130
• Protein ID: WP_013055004.1
• Coordinates: 200856..201206 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: K8Z47_RS01125
• Protein ID: WP_013055003.1
• Coordinates: 200570..200851 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z47_RS01100	195936..196907	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
K8Z47_RS01105	196916..197518	-	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
K8Z47_RS01110	197583..197948	+	366	WP_028411881.1	holo-ACP synthase	-
K8Z47_RS01115	198007..199065	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
K8Z47_RS01120	199179..200369	+	1191	WP_034327253.1	alanine racemase	-
K8Z47_RS01125	200570..200851	+	282	WP_013055003.1	hypothetical protein	Antitoxin
K8Z47_RS01130	200856..201206	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K8Z47_RS01135	201364..202200	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
K8Z47_RS01140	202203..202559	+	357	WP_013055006.1	STAS domain-containing protein	-
K8Z47_RS01145	202563..202964	+	402	WP_013055007.1	anti-sigma regulatory factor	-
K8Z47_RS01150	202996..203988	+	993	WP_223260212.1	PP2C family protein-serine/threonine phosphatase	-
K8Z47_RS01155	204048..204380	+	333	WP_013055009.1	anti-sigma factor antagonist	-
K8Z47_RS01160	204377..204862	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
K8Z47_RS01165	204828..205622	+	795	WP_028411878.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T216236 WP_013055004.1 NZ_CP083269:200856-201206 [Priestia aryabhattai]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T216236 NZ_CP112997:710213-710614 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT216236 WP_013055003.1 NZ_CP083269:200570-200851 [Priestia aryabhattai]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT216236 NZ_CP112997:709989-710216 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9