Detailed information of TA system TA216234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 91811..92436
• Replicon: plasmid p1
• Accession: NZ_CP083264
• Organism: Escherichia coli strain JNU

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K6T58_RS24040
• Protein ID: WP_000911324.1
• Coordinates: 91811..92209 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: K6T58_RS24045
• Protein ID: WP_000450532.1
• Coordinates: 92209..92436 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T58_RS24025 (88119)	88119..88640	+	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-
K6T58_RS24030 (88665)	88665..89396	+	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
K6T58_RS24035 (89649)	89649..91802	+	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
K6T58_RS24040 (91811)	91811..92209	-	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6T58_RS24045 (92209)	92209..92436	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..102134	102134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T216234 WP_000911324.1 NZ_CP083264:c92209-91811 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T216234 NZ_CP112997:c696774-696361 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT216234 WP_000450532.1 NZ_CP083264:c92436-92209 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT216234 NZ_CP112997:c697007-696771 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5