Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 15087..15730 Replicon plasmid p1
Accession NZ_CP083264
Organism Escherichia coli strain JNU

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K6T58_RS23620 Protein ID WP_001044768.1
Coordinates 15087..15503 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K6T58_RS23625 Protein ID WP_001261287.1
Coordinates 15500..15730 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T58_RS23605 (11586) 11586..12176 - 591 WP_000194578.1 hypothetical protein -
K6T58_RS23610 (12176) 12176..12433 - 258 WP_000371891.1 hypothetical protein -
K6T58_RS23615 (12787) 12787..14925 + 2139 WP_000350638.1 AAA family ATPase -
K6T58_RS23620 (15087) 15087..15503 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K6T58_RS23625 (15500) 15500..15730 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6T58_RS23630 (16026) 16026..16316 + 291 WP_000111771.1 hypothetical protein -
K6T58_RS23635 (16306) 16306..17205 + 900 WP_000963206.1 nucleotide-binding protein -
K6T58_RS23640 (17255) 17255..19480 - 2226 WP_000698737.1 P-loop NTPase fold protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..102134 102134


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T216229 WP_001044768.1 NZ_CP083264:c15503-15087 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T216229 NZ_CP112997:362615-362917 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT216229 WP_001261287.1 NZ_CP083264:c15730-15500 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT216229 NZ_CP112997:362965-363186 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCGCGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References