Detailed information of TA system TA216228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4484410..4485035
• Replicon: chromosome
• Accession: NZ_CP083263
• Organism: Escherichia coli strain JNU

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K6T58_RS21715
• Protein ID: WP_000911330.1
• Coordinates: 4484637..4485035 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: K6T58_RS21710
• Protein ID: WP_000450524.1
• Coordinates: 4484410..4484637 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T58_RS21685 (4480213)	4480213..4480683	-	471	WP_223675554.1	thioredoxin-dependent thiol peroxidase	-
K6T58_RS21690 (4480683)	4480683..4481255	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
K6T58_RS21695 (4481401)	4481401..4482279	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
K6T58_RS21700 (4482296)	4482296..4483330	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
K6T58_RS21705 (4483543)	4483543..4484256	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
K6T58_RS21710 (4484410)	4484410..4484637	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K6T58_RS21715 (4484637)	4484637..4485035	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6T58_RS21720 (4485182)	4485182..4486045	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
K6T58_RS21725 (4486060)	4486060..4488075	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
K6T58_RS21730 (4488149)	4488149..4488847	+	699	WP_000679823.1	esterase	-
K6T58_RS21735 (4488957)	4488957..4489157	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T216228 WP_000911330.1 NZ_CP083263:4484637-4485035 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T216228 NZ_CP112997:362615-362917 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT216228 WP_000450524.1 NZ_CP083263:4484410-4484637 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT216228 NZ_CP112997:362391-362618 [Mycobacterium tuberculosis variant bovis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33