Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4060344..4060998 Replicon chromosome
Accession NZ_CP083263
Organism Escherichia coli strain JNU

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K6T58_RS19695 Protein ID WP_000244772.1
Coordinates 4060591..4060998 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K6T58_RS19690 Protein ID WP_000354046.1
Coordinates 4060344..4060610 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T58_RS19665 (4055513) 4055513..4056256 + 744 WP_000951964.1 SDR family oxidoreductase -
K6T58_RS19670 (4056313) 4056313..4057746 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
K6T58_RS19675 (4057791) 4057791..4058102 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K6T58_RS19680 (4058266) 4058266..4058925 + 660 WP_000250274.1 hemolysin III family protein -
K6T58_RS19685 (4059121) 4059121..4060101 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K6T58_RS19690 (4060344) 4060344..4060610 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K6T58_RS19695 (4060591) 4060591..4060998 + 408 WP_000244772.1 protein YgfX Toxin
K6T58_RS19700 (4061038) 4061038..4061559 - 522 WP_001055874.1 flavodoxin FldB -
K6T58_RS19705 (4061671) 4061671..4062567 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K6T58_RS19710 (4062592) 4062592..4063302 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K6T58_RS19715 (4063308) 4063308..4065041 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T216226 WP_000244772.1 NZ_CP083263:4060591-4060998 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216226 NZ_CP112996:c3756186-3755794 [Mycobacterium tuberculosis variant bovis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216226 WP_000354046.1 NZ_CP083263:4060344-4060610 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216226 NZ_CP112996:c3756461-3756186 [Mycobacterium tuberculosis variant bovis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References