Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1794771..1795389 Replicon chromosome
Accession NZ_CP083263
Organism Escherichia coli strain JNU

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K6T58_RS08800 Protein ID WP_001291435.1
Coordinates 1795171..1795389 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K6T58_RS08795 Protein ID WP_000344800.1
Coordinates 1794771..1795145 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T58_RS08785 (1789860) 1789860..1791053 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6T58_RS08790 (1791076) 1791076..1794225 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K6T58_RS08795 (1794771) 1794771..1795145 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K6T58_RS08800 (1795171) 1795171..1795389 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K6T58_RS08805 (1795560) 1795560..1796111 + 552 WP_000102578.1 maltose O-acetyltransferase -
K6T58_RS08810 (1796227) 1796227..1796697 + 471 WP_000136192.1 YlaC family protein -
K6T58_RS08815 (1796861) 1796861..1798411 + 1551 WP_073514768.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K6T58_RS08820 (1798453) 1798453..1798806 - 354 WP_000878141.1 DUF1428 family protein -
K6T58_RS08830 (1799185) 1799185..1799496 + 312 WP_000409911.1 MGMT family protein -
K6T58_RS08835 (1799527) 1799527..1800099 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216218 WP_001291435.1 NZ_CP083263:1795171-1795389 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216218 NZ_CP112996:c3071674-3071318 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216218 WP_000344800.1 NZ_CP083263:1794771-1795145 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216218 NZ_CP112996:c3071888-3071658 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References