Detailed information of TA system TA216198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 773276..773805
• Replicon: chromosome
• Accession: NZ_CP083259
• Organism: Staphylococcus aureus strain HL25274

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3A98_RS03775
• Protein ID: WP_000621175.1
• Coordinates: 773443..773805 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K3A98_RS03770
• Protein ID: WP_000948331.1
• Coordinates: 773276..773446 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3A98_RS03740 (768313)	768313..768873	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
K3A98_RS03745 (769081)	769081..769560	+	480	WP_001287077.1	hypothetical protein	-
K3A98_RS03750 (769553)	769553..771136	+	1584	WP_001294637.1	PH domain-containing protein	-
K3A98_RS03755 (771123)	771123..771614	+	492	WP_001205908.1	PH domain-containing protein	-
K3A98_RS03760 (771618)	771618..771977	+	360	WP_000581197.1	holo-ACP synthase	-
K3A98_RS03765 (772043)	772043..773191	+	1149	WP_001281140.1	alanine racemase	-
K3A98_RS03770 (773276)	773276..773446	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K3A98_RS03775 (773443)	773443..773805	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3A98_RS03780 (774155)	774155..775156	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K3A98_RS03785 (775275)	775275..775601	+	327	WP_001052491.1	anti-sigma factor antagonist	-
K3A98_RS03790 (775603)	775603..776082	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
K3A98_RS03795 (776057)	776057..776827	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T216198 WP_000621175.1 NZ_CP083259:773443-773805 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T216198 NZ_CP112996:c1937225-1936836 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT216198 WP_000948331.1 NZ_CP083259:773276-773446 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT216198 NZ_CP112996:c1937449-1937222 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1