Detailed information of TA system TA216195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 1019413..1019968
• Replicon: chromosome
• Accession: NZ_CP083257
• Organism: Bifidobacterium longum subsp. longum strain BCBL-583

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BCBL_RS04285
• Protein ID: WP_077149462.1
• Coordinates: 1019413..1019736 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2ZDC4
• Locus tag: BCBL_RS04290
• Protein ID: WP_003829112.1
• Coordinates: 1019723..1019968 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCBL_RS04265 (BCBL_0865)	1015489..1016754	-	1266	WP_223616808.1	Fic family protein	-
BCBL_RS04270 (BCBL_0866)	1017298..1017663	-	366	WP_193641223.1	YccF domain-containing protein	-
BCBL_RS04275 (BCBL_0867)	1017901..1018488	+	588	WP_003833462.1	class I SAM-dependent methyltransferase	-
BCBL_RS04280 (BCBL_0868)	1018582..1019196	+	615	WP_223616810.1	cupin domain-containing protein	-
BCBL_RS04285 (BCBL_0869)	1019413..1019736	-	324	WP_077149462.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCBL_RS04290 (BCBL_0870)	1019723..1019968	-	246	WP_003829112.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BCBL_RS04295 (BCBL_0871)	1020142..1021398	+	1257	WP_003829116.1	HipA domain-containing protein	-
BCBL_RS04300 (BCBL_0872)	1021821..1023092	-	1272	WP_223616812.1	MFS transporter	-
BCBL_RS04305 (BCBL_0873)	1023249..1023935	-	687	WP_223616814.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11873.70 Da
• Isoelectric Point: 5.0771

>T216195 WP_077149462.1 NZ_CP083257:c1019736-1019413 [Bifidobacterium longum subsp. longum]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRILGLL

Nucleotide

• Length: 324 bp

>T216195 NZ_CP112996:c1575280-1574879 [Mycobacterium tuberculosis variant bovis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9339.43 Da
• Isoelectric Point: 4.5881

>AT216195 WP_003829112.1 NZ_CP083257:c1019968-1019723 [Bifidobacterium longum subsp. longum]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT216195 NZ_CP112996:c1575534-1575277 [Mycobacterium tuberculosis variant bovis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A087B6Q6