Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1551349..1552487 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name hipA Uniprot ID A0A454A433
Locus tag ECP_RS07645 Protein ID WP_001125435.1
Coordinates 1551349..1552221 (-) Length 291 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag ECP_RS07650 Protein ID WP_001304368.1
Coordinates 1552221..1552487 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS07625 1546641..1548920 - 2280 WP_000726723.1 acid resistance putative oxidoreductase YdeP -
ECP_RS07630 1549215..1549493 - 279 Protein_1451 type 1 fimbrial protein -
ECP_RS07635 1549507..1550313 - 807 Protein_1452 fimbrial biogenesis outer membrane usher protein -
ECP_RS07640 1550384..1551055 + 672 WP_000588930.1 LysR family transcriptional regulator -
ECP_RS07645 1551349..1552221 - 873 WP_001125435.1 HipA N-terminal domain-containing protein Toxin
ECP_RS07650 1552221..1552487 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
ECP_RS07655 1552795..1553553 + 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
ECP_RS07660 1553557..1554471 - 915 WP_001298637.1 bestrophin family protein -
ECP_RS07670 1554668..1556119 - 1452 WP_000854642.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1543275..1552505 9230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(119-251)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 291 a.a.        Molecular weight: 32608.75 Da        Isoelectric Point: 9.4223

>T21618 WP_001125435.1 NC_008253:c1552221-1551349 [Escherichia coli 536]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVMAFLMGSSEALKDRYDFMKFQVFQWLIGATDG
HAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHE
ILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 873 bp

>T21618 NC_008253:c1552221-1551349 [Escherichia coli 536]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTTATGGCTTTTTTAATGG
GGTCCAGCGAGGCGCTGAAAGATCGCTATGATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGC
CATGCAAAAAACTTCTCCGTATTTATTCAGGCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATT
TCCGGTCCTTGGCGGTACGGGAATACACATCAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAA
CGGCAATCGATAAAATTTATCCGCGACATTTTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAA
ATCCTGAGTGACTTTGCCAGAATGATTCCGGCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAA
CGTGGTGACGGCAGTTGAAACCAATGTGTTGAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT21618 WP_001304368.1 NC_008253:c1552487-1552221 [Escherichia coli 536]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT21618 NC_008253:c1552487-1552221 [Escherichia coli 536]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAATTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
GCAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A433

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References