Detailed information of TA system TA216178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 48171..48788
• Replicon: plasmid unnamed
• Accession: NZ_CP083180
• Organism: Enterococcus faecium strain H7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K9O36_RS12500
• Protein ID: WP_229204869.1
• Coordinates: 48636..48788 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9O36_RS12495
• Protein ID: WP_086291385.1
• Coordinates: 48171..48602 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O36_RS12465	43175..43630	-	456	Protein_42	hypothetical protein	-
K9O36_RS12470	43792..44531	-	740	Protein_43	transposase	-
K9O36_RS12475	44684..44866	-	183	WP_218569205.1	PcfJ domain-containing protein	-
K9O36_RS12480	45344..46292	+	949	Protein_45	IS30 family transposase	-
K9O36_RS12485	46334..46829	+	496	Protein_46	recombinase family protein	-
K9O36_RS12490	46927..47694	+	768	WP_086291384.1	CPBP family intramembrane metalloprotease	-
K9O36_RS12495	48171..48602	-	432	WP_086291385.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K9O36_RS12500	48636..48788	-	153	WP_229204869.1	type II toxin-antitoxin system HicA family toxin	Toxin
K9O36_RS12505	48784..49092	-	309	WP_233976340.1	hypothetical protein	-
K9O36_RS12510	49108..49308	-	201	WP_233976328.1	hypothetical protein	-
K9O36_RS12515	49472..50179	-	708	WP_233976329.1	hypothetical protein	-
K9O36_RS12520	50172..50609	-	438	WP_010776932.1	hypothetical protein	-
K9O36_RS12525	50625..50876	-	252	WP_010776931.1	hypothetical protein	-
K9O36_RS12530	50876..51082	-	207	WP_233976330.1	DNA helicase UvrA	-
K9O36_RS12535	51529..51789	-	261	WP_233976331.1	hypothetical protein	-
K9O36_RS12540	51963..52067	+	105	WP_008274030.1	putative holin-like toxin	-
K9O36_RS12545	52208..52693	-	486	WP_233976332.1	single-stranded DNA-binding protein	-
K9O36_RS12550	52696..53553	-	858	WP_086311938.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..124878	124878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5727.49 Da
• Isoelectric Point: 9.9282

>T216178 WP_229204869.1 NZ_CP083180:c48788-48636 [Enterococcus faecium]
IEKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLEQKILKDAGLK

Nucleotide

• Length: 153 bp

>T216178 NZ_CP112996:c698715-698302 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15825.95 Da
• Isoelectric Point: 4.1719

>AT216178 WP_086291385.1 NZ_CP083180:c48602-48171 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEELLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT216178 NZ_CP112996:c698948-698712 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA