216177

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 501798..502369 Replicon chromosome
Accession NZ_CP083179
Organism Enterococcus faecium strain H7

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag K9O36_RS02390 Protein ID WP_002286801.1
Coordinates 502028..502369 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag K9O36_RS02385 Protein ID WP_002323011.1
Coordinates 501798..502028 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O36_RS02360 496918..498249 + 1332 WP_233976267.1 FAD-containing oxidoreductase -
K9O36_RS02365 498271..498897 + 627 WP_081139837.1 cysteine hydrolase -
K9O36_RS02370 499080..499661 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
K9O36_RS02375 500393..500968 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
K9O36_RS02380 501173..501511 - 339 WP_002286804.1 hypothetical protein -
K9O36_RS02385 501798..502028 + 231 WP_002323011.1 hypothetical protein Antitoxin
K9O36_RS02390 502028..502369 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K9O36_RS02395 503219..503404 + 186 WP_002304207.1 hypothetical protein -
K9O36_RS02400 503909..504103 + 195 WP_002295146.1 hypothetical protein -
K9O36_RS02405 504294..504543 + 250 Protein_478 transposase -
K9O36_RS02410 504787..505617 - 831 WP_002286789.1 manganese catalase family protein -
K9O36_RS02415 505825..507159 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T216177 WP_002286801.1 NZ_CP083179:502028-502369 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T216177 NZ_CP112996:c696379-695987 [Mycobacterium tuberculosis variant bovis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT216177 WP_002323011.1 NZ_CP083179:501798-502028 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT216177 NZ_CP112996:c696633-696376 [Mycobacterium tuberculosis variant bovis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References