Detailed information of TA system TA216174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1028457..1028950
• Replicon: chromosome
• Accession: NZ_CP083176
• Organism: Gardnerella swidsinskii strain JNFY3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: K9E37_RS04015
• Protein ID: WP_240613512.1
• Coordinates: 1028457..1028669 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2RD11
• Locus tag: K9E37_RS04020
• Protein ID: WP_012913778.1
• Coordinates: 1028663..1028950 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9E37_RS03980 (K9E37_03960)	1023598..1024197	+	600	WP_103013773.1	FMN-binding protein	-
K9E37_RS03985 (K9E37_03965)	1024210..1024446	-	237	WP_162861111.1	hypothetical protein	-
K9E37_RS03990 (K9E37_03970)	1024629..1025132	-	504	WP_248893077.1	hypothetical protein	-
K9E37_RS03995 (K9E37_03975)	1025327..1026067	+	741	WP_248893079.1	response regulator transcription factor	-
K9E37_RS04000 (K9E37_03980)	1026061..1027278	+	1218	WP_248893081.1	histidine kinase dimerization/phosphoacceptor domain-containing protein	-
K9E37_RS04005 (K9E37_03985)	1027317..1027577	-	261	WP_012913776.1	hypothetical protein	-
K9E37_RS04010 (K9E37_03990)	1027767..1027979	-	213	WP_004109881.1	hypothetical protein	-
K9E37_RS04015 (K9E37_03995)	1028457..1028669	+	213	WP_240613512.1	addiction module killer protein	Toxin
K9E37_RS04020 (K9E37_04000)	1028663..1028950	+	288	WP_012913778.1	putative addiction module antidote protein	Antitoxin
K9E37_RS04025 (K9E37_04005)	1029162..1029755	-	594	WP_041160459.1	hypothetical protein	-
K9E37_RS04030 (K9E37_04010)	1030123..1031202	-	1080	WP_012913780.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
K9E37_RS04035 (K9E37_04015)	1031285..1033492	-	2208	WP_076003126.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1022708..1043929	21221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (6-64)

Antitoxin

• Uncharacterized protein HI1420 (4-90)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7716.13 Da
• Isoelectric Point: 10.2197

>T216174 WP_240613512.1 NZ_CP083176:1028457-1028669 [Gardnerella swidsinskii]
IAGKPVGDLHPVCNGISELRFTFGPGYRVYFAQKGLKLMLLLAGGDKSSQSKDIKQAKILLAQLIEEKKW

Nucleotide

• Length: 213 bp

>T216174 NZ_CP112996:365112-365537 [Mycobacterium tuberculosis variant bovis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10358.74 Da
• Isoelectric Point: 4.8283

>AT216174 WP_012913778.1 NZ_CP083176:1028663-1028950 [Gardnerella swidsinskii]
MVNISKWDASEYLEDDDDVIAYLNAAAELNDPRLLQAAIGDIAKARGMKEIAQKAEVGRESLYKSLRRDGNPSFQTIAKV
VRALGGRIAIEPANA

Nucleotide

• Length: 288 bp

>AT216174 NZ_CP112996:364894-365115 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCGCGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: D2RD11