Detailed information of TA system TA216173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-HTH_XRE
• Location: 376207..377507
• Replicon: chromosome
• Accession: NZ_CP083175
• Organism: Gardnerella vaginalis strain JNFY4

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K9E38_RS01665
• Protein ID: WP_029600575.1
• Coordinates: 376207..376995 (-)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: S4HGB9
• Locus tag: K9E38_RS01670
• Protein ID: WP_004113003.1
• Coordinates: 377034..377507 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9E38_RS01645 (K9E38_01650)	372109..372846	+	738	WP_004112993.1	thiamine diphosphokinase	-
K9E38_RS01650 (K9E38_01655)	373022..374077	+	1056	WP_004112995.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
K9E38_RS01655 (K9E38_01660)	374188..374688	+	501	WP_004112997.1	Cys-tRNA(Pro) deacylase	-
K9E38_RS01660 (K9E38_01665)	374736..375695	+	960	WP_004112999.1	aldose 1-epimerase family protein	-
K9E38_RS01665 (K9E38_01670)	376207..376995	-	789	WP_029600575.1	zeta toxin family protein	Toxin
K9E38_RS01670 (K9E38_01675)	377034..377507	-	474	WP_004113003.1	helix-turn-helix transcriptional regulator	Antitoxin
K9E38_RS01675 (K9E38_01680)	378045..379520	-	1476	WP_248902617.1	hypothetical protein	-
K9E38_RS01680 (K9E38_01685)	380379..381389	+	1011	WP_004116755.1	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-59)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 30152.79 Da
• Isoelectric Point: 9.1040

>T216173 WP_029600575.1 NZ_CP083175:c376995-376207 [Gardnerella vaginalis]
MVIEDFSEEYFTHCLLRNLRSLQMYKHADLHPKAVLLGGQSGAGKTTIHVIKQKEFNGNIIILDADSYRTQHPYYCELLD
KYGKESVKYTSAFSAKMVRSLIDELSSKKYNLIIEGTLRSVEVPSSTAKMLIDRGYRISLAVIATKPELSWISTLIRYRE
MYLEDPKSARATPKDHHDNIVNNIVDNLLKLEQSKIFDRIQIYKRDEKCIYDSDSYKNSPNITAASVLKEVLFGKKTIDE
KKLICHAKNRLNELDKLIDKSL

Nucleotide

• Length: 789 bp

>T216173 NZ_CP112996:364544-364846 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 18016.48 Da
• Isoelectric Point: 5.3394

>AT216173 WP_004113003.1 NZ_CP083175:c377507-377034 [Gardnerella vaginalis]
MVSVNIRALRRSKKITQQELAEALGVSRNSIVRYENGTSAISTRLIDKICDKFKVSLFDIVHEKELSASDKYDLNMKIEV
LKERGALLLARLYKYEDSMNIQFDDSSNAWIAISDDLSDVINTKIYTSTNFEDINRYIGYLDGIERLLEYVSCNKNS

Nucleotide

• Length: 474 bp

>AT216173 NZ_CP112996:364894-365115 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCGCGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: S4HGB9