Detailed information of TA system TA216172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-HTH_XRE
• Location: 1296282..1297582
• Replicon: chromosome
• Accession: NZ_CP083174
• Organism: Gardnerella vaginalis strain JNFY9

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K9E39_RS05035
• Protein ID: WP_029600575.1
• Coordinates: 1296794..1297582 (+)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: S4HGB9
• Locus tag: K9E39_RS05030
• Protein ID: WP_004113003.1
• Coordinates: 1296282..1296755 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9E39_RS05010 (K9E39_05020)	1291777..1291980	-	204	WP_248912108.1	hypothetical protein	-
K9E39_RS05015 (K9E39_05025)	1292018..1292341	+	324	WP_004114523.1	hypothetical protein	-
K9E39_RS05020 (K9E39_05030)	1292501..1293511	-	1011	WP_004113007.1	UDP-glucose 4-epimerase GalE	-
K9E39_RS05025 (K9E39_05035)	1294370..1295746	+	1377	WP_101892056.1	hypothetical protein	-
K9E39_RS05030 (K9E39_05040)	1296282..1296755	+	474	WP_004113003.1	helix-turn-helix transcriptional regulator	Antitoxin
K9E39_RS05035 (K9E39_05045)	1296794..1297582	+	789	WP_029600575.1	zeta toxin family protein	Toxin
K9E39_RS05040 (K9E39_05050)	1298092..1299051	-	960	WP_004112999.1	aldose 1-epimerase family protein	-
K9E39_RS05045 (K9E39_05055)	1299099..1299599	-	501	WP_004112997.1	Cys-tRNA(Pro) deacylase	-
K9E39_RS05050 (K9E39_05060)	1299710..1300765	-	1056	WP_004112995.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
K9E39_RS05055 (K9E39_05065)	1300941..1301678	-	738	WP_004112993.1	thiamine diphosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-59)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 30152.79 Da
• Isoelectric Point: 9.1040

>T216172 WP_029600575.1 NZ_CP083174:1296794-1297582 [Gardnerella vaginalis]
MVIEDFSEEYFTHCLLRNLRSLQMYKHADLHPKAVLLGGQSGAGKTTIHVIKQKEFNGNIIILDADSYRTQHPYYCELLD
KYGKESVKYTSAFSAKMVRSLIDELSSKKYNLIIEGTLRSVEVPSSTAKMLIDRGYRISLAVIATKPELSWISTLIRYRE
MYLEDPKSARATPKDHHDNIVNNIVDNLLKLEQSKIFDRIQIYKRDEKCIYDSDSYKNSPNITAASVLKEVLFGKKTIDE
KKLICHAKNRLNELDKLIDKSL

Nucleotide

• Length: 789 bp

>T216172 NZ_CP112996:364544-364846 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 18016.48 Da
• Isoelectric Point: 5.3394

>AT216172 WP_004113003.1 NZ_CP083174:1296282-1296755 [Gardnerella vaginalis]
MVSVNIRALRRSKKITQQELAEALGVSRNSIVRYENGTSAISTRLIDKICDKFKVSLFDIVHEKELSASDKYDLNMKIEV
LKERGALLLARLYKYEDSMNIQFDDSSNAWIAISDDLSDVINTKIYTSTNFEDINRYIGYLDGIERLLEYVSCNKNS

Nucleotide

• Length: 474 bp

>AT216172 NZ_CP112996:364320-364547 [Mycobacterium tuberculosis variant bovis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: S4HGB9