Detailed information of TA system TA216171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-HTH_XRE
• Location: 1331393..1332693
• Replicon: chromosome
• Accession: NZ_CP083172
• Organism: Gardnerella vaginalis strain JNFY13

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K9E41_RS05240
• Protein ID: WP_029600575.1
• Coordinates: 1331905..1332693 (+)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: S4HGB9
• Locus tag: K9E41_RS05235
• Protein ID: WP_004113003.1
• Coordinates: 1331393..1331866 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9E41_RS05225 (K9E41_05245)	1327570..1328580	-	1011	WP_004116755.1	UDP-glucose 4-epimerase GalE	-
K9E41_RS05230 (K9E41_05250)	1329439..1330857	+	1419	WP_248903037.1	hypothetical protein	-
K9E41_RS05235 (K9E41_05255)	1331393..1331866	+	474	WP_004113003.1	helix-turn-helix transcriptional regulator	Antitoxin
K9E41_RS05240 (K9E41_05260)	1331905..1332693	+	789	WP_029600575.1	zeta toxin family protein	Toxin
K9E41_RS05245 (K9E41_05265)	1333202..1334161	-	960	WP_004112999.1	aldose 1-epimerase family protein	-
K9E41_RS05250 (K9E41_05270)	1334209..1334709	-	501	WP_004112997.1	Cys-tRNA(Pro) deacylase	-
K9E41_RS05255 (K9E41_05275)	1334820..1335875	-	1056	WP_004112995.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
K9E41_RS05260 (K9E41_05280)	1336051..1336788	-	738	WP_004112993.1	thiamine diphosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-59)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 30152.79 Da
• Isoelectric Point: 9.1040

>T216171 WP_029600575.1 NZ_CP083172:1331905-1332693 [Gardnerella vaginalis]
MVIEDFSEEYFTHCLLRNLRSLQMYKHADLHPKAVLLGGQSGAGKTTIHVIKQKEFNGNIIILDADSYRTQHPYYCELLD
KYGKESVKYTSAFSAKMVRSLIDELSSKKYNLIIEGTLRSVEVPSSTAKMLIDRGYRISLAVIATKPELSWISTLIRYRE
MYLEDPKSARATPKDHHDNIVNNIVDNLLKLEQSKIFDRIQIYKRDEKCIYDSDSYKNSPNITAASVLKEVLFGKKTIDE
KKLICHAKNRLNELDKLIDKSL

Nucleotide

• Length: 789 bp

>T216171 NZ_CP112996:71848-72249 [Mycobacterium tuberculosis variant bovis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 18016.48 Da
• Isoelectric Point: 5.3394

>AT216171 WP_004113003.1 NZ_CP083172:1331393-1331866 [Gardnerella vaginalis]
MVSVNIRALRRSKKITQQELAEALGVSRNSIVRYENGTSAISTRLIDKICDKFKVSLFDIVHEKELSASDKYDLNMKIEV
LKERGALLLARLYKYEDSMNIQFDDSSNAWIAISDDLSDVINTKIYTSTNFEDINRYIGYLDGIERLLEYVSCNKNS

Nucleotide

• Length: 474 bp

>AT216171 NZ_CP112996:71616-71855 [Mycobacterium tuberculosis variant bovis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGTAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Antitoxin

• AlphaFold DB: S4HGB9